Nitrogen Fertilization and Inoculation of Seeds with Rhizobium tropici on the Agronomic Performance of Common Beans

Main Article Content

Darlan Capelesso
Antonio Carlos Torres da Costa
José Barbosa Duarte Júnior

Abstract

Aims: This study aimed to evaluate the influence of nitrogen fertilization and inoculation of seeds with Rhizobium tropici on the agronomic performance of common bean (Phaseolus vulgaris L.).

Study Design: The experimental design consisted of a randomized block in a 4x2 factorial layout, with 4 replicates, the first factor referring to inoculant doses (0, 50, 100 and 200 mL for each 25 kg of seed), while the second refers to nitrogen fertilization (0 and 40 kg ha of N).

Place and Duration of Study: The study was conducted to the field in a no-till system area, in the southwestern region of Paraná, Brazil. The soil is classified as a Purple Latosol, with a clayey texture.

Methodology: The adopted spacing was 0.45 m between rows, and the seeding density used was 12 seeds per furrow meter. The inoculants NITRO 1000 Rhizobium tropici SEMIA 4077 and SEMIA 4088 were applied, varied according to the treatments. The cultivar used was IPR – Tangará.

Results: As for grain yield, it was observed that nitrogen fertilization did not contribute to the increase in grain yield, and higher productivity was obtained in the absence of N. This was probably due to the high content of organic matter present in the soil.

Conclusion: Nitrogen fertilization at sowing and seed inoculation with Rhizobium tropici did not influence the plant population and the 1000-grain mass. In the absence of nitrogen fertilization at sowing, pods with a longer length and higher grain yield were obtained. Inoculation of the seeds with Rhizobium tropici exerts a positive influence on plant height, number of nodes of the main stem, number of pods per plant, and number of beans per pod, the dose recommended by the manufacturer (100 mL) being efficient, with the possibility of applying a dose of 50 mL, to satisfactory results.

Keywords:
Phaseolus vulgaris L., biological nitrogen fixation, inoculation, IPR-Tangará.

Article Details

How to Cite
Capelesso, D., Costa, A. C. T. da, & Júnior, J. B. D. (2019). Nitrogen Fertilization and Inoculation of Seeds with Rhizobium tropici on the Agronomic Performance of Common Beans. Journal of Experimental Agriculture International, 40(1), 1-9. https://doi.org/10.9734/jeai/2019/v40i130354
Section
Original Research Article

References

National Supply Company. Follow-up of the Brazilian Grain Harvest. Safra 2017/18. 9º Survey. Brasília: Conab. 2018;1-178. (Accessed 01 July 2018)
Available:https://www.conab.gov.br/

War AF, Silva DB, Rodrigues GC. Management of irrigation and nitrogen fertilization for common bean in the Cerrado region. Pesquisa Agropecuária Brasileira. 2000;35(6):1229-36. English.
DOI:http://dx.doi.org/10.1590/S0100-204X2000000600020

Salgado FHM, Silva J, Oliveira TC, Barros HB, NG Steps, Fidelis RR. Efficiency of common bean genotypes in response to nitrogen fertilization. Tropical Agriculture Research. 2012;42(4):368-74. English.
DOI: http://dx.doi.org/10.1590/S1983-40632012000400007

Vieira RF, Lima MS, Neves JCL, Andrade MJB. Fertilizing. In: Carneiro JE, Paula Júnior TJ, Borém A. editors. Beans from planting to harvest. Viçosa: Editora UFV; 2015.

Pelegrin R, Merchant FM, Otsubo IMN, Otsubo AA. Bean culture response to nitrogen fertilization and rhizobium inoculation. Brazilian Journal of Soil Science. 2009;33(1):2219-26. English.
DOI: http://dx.doi.org/10.1590/S0100-06832009000100023

Macdonald GK, Bennett EM, Potter PA, Rramankutty N. Agronomic phosphorus imbalances across the world's croplands. Proceedings of the National Academy of Sciences of the United States of America. 2011;108(7):3086-91.
DOI: 10.1073 / pnas.1010808108

Foley JA, Ramankutty N, Brauman KA, Cassidy ES, Gerber JS, Johnston M, Mueller ND, O'connell C, Ray DK, West PC, Balzer C, Bennett EM, Carpenter SR, Hill J, Monfreda C, Polasky S, Rockstrom J, Sheehan J, Siebert S, Tilman D, Zaks DPM. Solutions for a cultivated planet. Nature. 2011;478(7369):337-42.
DOI: 10.1038 / nature10452

Rabelo ACR, Ribeiro DF, Rezende RM, Alcantara E, Freitas AS. Nitrogen fertilization on bean crop. Journal of the Vale do Rio Verde University. 2017;15(1): 825-41. English.

Caviglione JH, Kilhl LRB, Caramori PH, Oliveira D. Climatic charts of Paraná. Londrina: Iapar; 2000.

Santos HG, Almeida JA, Oliviera JB, Lumbreras JF, Ljos Anjos, Coelho MR, Jacomine PKT, Cunha TJF, Oliveira VA. Brazilian system of soil classification. 3rd ed. Brasília: Embrapa; 2013.

Federal University of Santa Maria. The seed and its germination.
(Accessed 18 August 2018)
Available:http://coral.ufsm.br/sementes

Andrade MJB, Oliveira DP, Figueiredo MA, Martins FAD. Edafoclimatic requirements. In: In: Carneiro JE, Paula Júnior TJ, Borém A. editors. Beans from planting to harvest. Viçosa: Editora UFV; 2015.

Araújo JLS, Stradiotto R, Franco AA. Selection of common bean cultivars (Phaseolus vulgaris L.) for biological fixation of nitrogen under high temperature conditions. In: 4th National Bean Research Meeting. Proceedings. Londrina: Iapar. 1993;136.

Ferreira AN, Arf O, Carvalho MAC, Araújo RS, Sá MS, Buzetti S. Rhizobium tropici strains on bean inoculation. Scientia Agricola. 2000;57(3):507-12. English.
DOI:http://dx.doi.org/10.1590/S0103-90162000000300021

Peres JRR, Suhet AR, Mendes IC, Vargas MAT. Effect of inoculation with rhizobium and nitrogen fertilization on seven bean cultivars on cerrado soil. Brazilian Journal of Soil Science. 1994;18(3):415-20. English.

Matoso SCG, Kusdra JF. Nodulation and bean growth in response to the application of molybdenum and rhizobial inoculant. Brazilian Journal of Agricultural and Environmental Engineering. 2014;18(6): 567-73. English.
DOI: http://dx.doi.org/10.1590/S1415-43662014000600001

New MCSS, Tanaka RT, Mascarenhas HAA. Nitrogen and potassium in symbiotic N fixation by soybean cultivated in winter. Scientia Agricola. 1999;56(1):143-56. English.
DOI: http://dx.doi.org/10.1590/S0103-90161999000100021

Souza EFC, Soratto RP, Pagani FA. Nitrogen application and inoculation with rhizobia in common bean cultivated after maize intercropped with brachiaria. Pesquisa Agropecuária Brasileira. 2011; 11(4):370-77. English.
DOI: http://dx.doi.org/10.1590/S0100-204X2011000400005

Soratto RP, Silva TRB, Arf O, Carvalho MAC. Levels and times of application of nitrogen in cover in common bean irrigated in no-tillage. Agronomic Culture. 2001; 10(1):89-99. English.

Alvarez ACC, Arf O, Alvarez RCF, Pereira JCR. Response of the bean to the application of doses and sources of nitrogen under cover in the no-tillage system. Acta Scientiarum Agronomy. 2005;27(1):69-75. English.
DOI:http://dx.doi.org/10.4025/actasciagron.v27i1.1927

Araujo FF, Carmona FG, Tiritan CS, Creste JE. Biological fixation of N in common bean submitted to inoculant dosages and chemical treatment in the seed compared to nitrogen fertilization. Acta Scientiarum Agronomy. 2007;29(4): 535-40. English.
DOI:http://dx.doi.org/10.4025/actasciagron.v29i4.416

Soratto RP, Carvalho MAC, Arf O. Chlorophyll content and yield of common bean due to nitrogen fertilization. Pesquisa Agropecuária Brasileira. 2004;39(9):895-901. English.
DOI:http://dx.doi.org/10.1590/S0100-204X2004000900009

Romanini Junior A, Arf O, Binotti FFS, Sá ME, Buzetti S, Fernandes FA. Evaluation of rhizobium inoculation and nitrogen fertilization in common bean development under no - tillage system. Bioscience Journal. 2007;23(4):74-82. English.

Binotti FFS. Nitrogen management in winter bean in succession to corn and Brachiaria under no - tillage system. 2009; 178. Thesis (Doctorate in Agronomy / Production Systems) - Faculty of Engineering. State University of São Paulo, Ilha Solteira; 2009.

Farinelli R, Lemos LB, Penariol FG, Egéa MM, Gasparoto MG. Coverage nitrogen fertilization in common bean and no - tillage. Pesquisa Agropecuária Brasileira. 2006;41(2):307-12. English.
DOI:http://dx.doi.org/10.1590/S0100-204X2006000200016