Biological, Serological and Molecular Characterisation of a New Virus Species Infecting Telfairia occidentalis in Calabar, Cross River State, Nigeria

Main Article Content

O. I. Eyong
A. T. Owolabi
A. A. J. Mofunanya
E. E. Ekpiken

Abstract

Telfairia occidentalis (Hook) belongs to the family Cucurbitaceae and is an economically important cash crop worldwide. It is widely cultivated in Nigeria including the South Eastern part of the country. This research was aimed at isolating, characterising and identifying a new virus species infecting Telfairia occidentalis in Calabar, Cross River State. Diagnostic tools employed included host range/symptomatology, insect transmission test, Antigen Coated Plate (ACP) Enzyme Linked-Immunosorbent Assay (ELISA) and gene sequencing. Results revealed that the virus isolate infected only members of the cucurbit family producing rugosity, mosaic, mottle and leaf malformation/deformation. The virus isolate was transmitted by Aphis spiraecola in a fore-gut manner and not by A. citricida. It reacted positively against universal potyvirus antiserum. Sequence analysis showed that the Telfairia occidentalis virus isolate had 75% sequence identity with Moroccan watermelon mosaic virus (MWM) which was found to be the closest. The virus was consequently considered a new species of potyvirus for which the name Telfairia severe mosaic virus (TeSMV) was suggested.

Keywords:
Telfairia occidentalis, ACP-ELISA, Potyvirus, Telfairia severe mosaic virus.

Article Details

How to Cite
Eyong, O. I., Owolabi, A. T., Mofunanya, A. A. J., & Ekpiken, E. E. (2020). Biological, Serological and Molecular Characterisation of a New Virus Species Infecting Telfairia occidentalis in Calabar, Cross River State, Nigeria. Journal of Experimental Agriculture International, 42(1), 23-33. https://doi.org/10.9734/jeai/2020/v42i130448
Section
Original Research Article

References

Akoroda MO. Ethnobotany of Telfairia occidentalis among Igbos of Nigeria. Economic Botany. 1990;29-39.

Mofunanya AAJ, Omokaro DN, Owolabi AT, Ine-Ibehe NE. Effect of Telfairia mosaic virus (TeMV) on the proximate, mineral and anti-nutritive contents of Telfairia occidentalis Hook (Fluted pumpkin). Nigerian Journal of Botany. 2008;21(30):304-315.

Sanjur O, Pierno D, Andres T, Wessel-Beaver L. Phylogenetic relationships among domesticated and wild species of cucumber inferred from a mitochondria gene. Implication for crop plant evolution and areas of origin. Proceedings of National Academy of Science USA. 2008;99:535-540.

Sitterly WR. Breeding for disease resistance in cucurbits. Annual Review of Phytopathology. 1972;10:471-490.

Peter C, David BV. Population dynamics of the pumpkin caterpillar, Diaphania indica (Saunders) (Lepidoptera: Pyralidae). Tropical Pest Management. 1991;37:75-79.

Duyck PF, David P, Quilici S. A review of relationships between interspecific competition and invasions in fruit flies (Diptera tephritidae). Ecological Entomology. 2004;29:511-520.

Dhillon MK, Singh R, Naresh JS, Sharma HC. The melon fruit fly, Bactrocera cucurbitae: A review of its biology and management. Journal of Insect Science. 2005;5:1-16.

HansPetersen HN, McSorley R, Liburd OE. The impact of intercropping squash with non-crop vegetation borders on the above-ground arthropod community. Florida Entomologist. 2010;93:590-608.

Brown JK, Frohlich DR, Rosell RC. The sweet potato or silverleaf whiteflies: Biotypes of Bemisia tabaci or a species complex? Annual Review of Entomology. 1995;40:511-34.

Morse JG, Hoddle MS. Invasion biology of thrips. Annual Review of Entomology. 2006;51:67-89.

Powell G, Tosh CR, Hardie J. Host plant selection by aphids: Behavioral, evolutionary and applied perspectives. Annual Review of Entomology. 2006;51: 309-330.

Provvidenti R. Disease caused by viruses. Compendium of cucurbits disease. Minnesota USA. American Phytopathology Society. St. Paul; 1996.

Kumar S. Methods for the diagnosis of plant virus disease. IITA Ibadan, Laboratory Manual; 2009.

Abarshi MM, Mohammed IU, Wasswa P, Hillocks RJ, Holt J, Legg JP, Seal SE, Maruthi MN. Optimization of diagnostic RT-PCR protocols and sampling procedures for the reliable and cost-effective detection of Cassava brown streak virus. Journal of Virology Methods. 2010;163(2):353-359.

Pappu S, Brand R, Pappu H, Rybicki E, Gough K, Frenkel M, Niblett C. A polymerase chain reaction method adapted for selective amplification and cloning of 3 sequence of potyviral genomes: Application to Dasheen mosaic virus. Journal of Virological Methods. 1993;41:9-20.

Maixner F, Wagner M, Lücker S, Pelletier E, Schmitz‐Esser S, Hace K, Spieck E, Konrat R, Le Paslier D, Daims H. Environmental genomics reveals a functional chlorite dismutase in the nitrite‐oxidizing bacterium ‘Candidatus Nitrospira defluvii’. Environmental Micro-biology. 2008;10(11):3043-56.
Available:http://www.ncbi.nlm.nih.gov/BLAST/

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution. 2013; 30(12):2725-2729.

Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution. 2007;24(8):1596-1599.

Roy G, Jain RK, Bhat AI. Comparative host range and serological studies of Papaya ringspot potyvirus isolates. Indian Phytopathology. 1999;52:14-17.

Lecoq H, Jane R. Zucchini yellow mosaic virus. Plant Patholology Journal. 2001;46: 809-829.

Mosahebil GH, Hosseini S, Koohi M, Okhovvat SM. Characterization of the Zucchini yellow mosaic virus from Squash in Tehran Province. Journal of Agricultural Science Technology. 2007;9:137-143.

Owolabi AT, Rabenstein F, Ehrig F. A strain of Papaya ringspot virus naturally infecting cucumber (Cucumis sativus L.) in Calabar, South Eastern Nigeria. Nigerian Journal of Botany. 2008;21(1):97-108.

Robertson E. Biological and molecular characterization of Morroccan watermelon mosaic virus and potyvirus isolate from Eastern Sudan. American Phytopatho-logical Society. 2001;85:547-552.

Desbiez C, Lecoq H. Zucchini yellow mosaic virus. Plant Pathology. 2012;109: 76-82.

Kumar S, Sankarlingam A, Rabindran R. Characterization and confirmation of Papaya ringspot virus-W strain infecting Trichosanthese cucumerina at Tamil Nadu, India. Journal of Plant Pathology & Microbiology. 2014;5:225.

Antignus Y, Wang M, Pearlsman O, Lachman N, Lavi Gal-On A. Biological and molecular characterization of a new cucurbit-infecting Tobamovirus. The American Phytopathological Society. 2017;154:51–59.

Yakoubi S, Desbiez C, Fakhfakh H, Wipf-Scheibel C, Marrakchi M, Lecoq H. Biological characterization and complete nucleotide sequence of a Tunisian isolate of Moroccan watermelon mosaic virus. Archives of Virology. 2008;153:117–125.

Ali A, Natsuaki T, Okuda S. Identification and molecular characterization of viruses infecting cucurbits in Oklahoma, USA. Journal of Phytopathology. 2004;152:677-682.

Hakan F. Occurrence of Alfalfa mosaic virus (AMV). Diseases on cucumber crops in Northern Cyprus. Acta Horticulturae. 2012;960:341-346.

Owolabi AT, Rabenstein F, Ehrigh F, Maiss E, Vetten. Strain of Morroccan watermelon mosaic virus isolated from Lagenaria breviflorus and Coccinia barteri in Calabar, Southern Nigeria. International Journal of Virology. 2012;8(3):258-270.

Owolabi AT, Ekpiken EE. Transmission efficiency of two strains of Morroccan watermelon mosaic virus by two clones of Aphis spiraecola (Patch). International Journal of Virology. 2014;10(4):253-262.

Somowiyarjo S, Sako N, Nonak F. Dot-immunobinding assay for Zucchini yellow mosaic virus using polyclonal and monoclonal antibodies. Annals of the Phytopathological Society of Japan. 1989;55:56–63.

Malaurie B, Trouslot MF, Berthaud J, Bousalem M, Pinel A, Dubern J. Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm. Electronic Journal of Biotechnology. 1998;1(3):103-117.

Cardoso FH, Armada A, Fonseca AM, Santos MT, Sequeira J, Clemente A, Sequeira O, Novo C. Sequences of the N-termini of coat proteins of Portuguese Zucchini yellow mosaic virus isolates and of an epitope recognized by a monoclonal antibody. Journal of Plant Pathology. 2005;87:229–232.

Shang H, Xie Y, Zhou X, Qian Y, Wu J. Monoclonal antibody-based serological methods for detection of Cucumber green mottle mosaic virus. Virology Journal. 2011;8:228.

Wulandari DR, Ermayanti TM. Detection of potyvirus using RT-PCR and ACP-ELISA of Dioscorea species and in vitro shoot multiplication of the virus free plants. Annales Bogoriences. 2011;15(2):56-78.

Wu JX, Ni YQ, Liu H, Ding M, Zhou XP. Monoclonal antibody-based serological assays and immunocapture RT-PCR for detecting Rice dwarf virus in field rice plants and leafhopper vectors. Journal of Virological Methods. 2014;195:134– 140.

Zhe C, Zhang M, Zhou X, Wu J. Development and detection application of monoclonal antibodies against Zucchini yellow mosaic virus. Journal of Intergrative Agriculture. 2017;16(1):115–124.

Daryono B, Somowiyarjo S, Natsuaki T. Biological and molecular characterization of melon-infecting Kyuri green mottle mosaic virus in Indonesia. Journal of Phytopathology. 2005;153:588–595.

Helaly HS, Ahmed AA, Awad MA, Soliman AM. Biological and molecular characterization of potato infecting Alfalfa mosaic virus in Egypt. International Journal of Virology. 2012;8:106-113.

Jain RK, Pappu SS, Varma A, Ram RD. Molecular characterization of Papaya ringspot potyvirus isolates from India. Annual Applied Biology. 2008;132:413-425.

Muharrem A, Cecile D, Asime FC. Characterization of an emerging isolate of Watermelon mosaic virus in Turkey. International Journal of Agriculture & Biology. 2009;17(1):211–215.

King AM, Lefkowitz E, Adams MJ, Carstens EB. Virus taxonomy: 9th Report of the International Committee on Taxonomy of Viruses. San Diego, Elsevier Academic Press; 2011.

Usher L, Sivparsad B, Gubba A. Isolation, identification and molecular characterisa-tion of an isolate of Watermelon mosaic virus 2 occurring in KwaZulu-Natal, South Africa. International Journal of Virology. 2012;18:10-17.

Shukla DD, Ward CW, Brunt AA. The Potyviridae. CAB International, Wallingford; 1994.

Frenkle MJ, Ward CW, Shukla DD. The use of 3’ non-coding nucleotide sequences in the taxonomy of potyviruses: Application to watermelon mosaic virus 2 and soybean mosaic virus-N. Journal of General Virology. 1989;70:2775-2783.