Vegetative Propagation of Himatanthus sucuuba (Spruce ex Müll. Arg.) Woodson by Stem Cuttings

Main Article Content

Rayssa Gomes Vasconcelos
Raquel da Silva Medeiros
Daniel da Silva
Paulo de Tarso Barbosa Sampaio


The vegetative propagation by cuttings can be an advantageous alternative to the production of Himatanthus sucuuba seedlings because it allows the obtention of a large number of plants genetically identical to the matrix plant in a shorter time and space. However, such a technique is simultaneously influenced by different factors that can compromise its success. In this sense, the present study aimed to evaluate the effect of different types of cuttings and indole-3-butyric acid (IBA) concentration, on the survival and rooting of cuttings of H. sucuuba. The experimental design used was in randomized complete blocks, with a factorial arrangement: 2 types of cuttings (with and without leaves) and 3 concentrations of IBA (0, 1000 and 3000 mg L–1). After 100 days, were evaluated the following variables: survival (%), rooting (%), number of roots per cutting, length of the largest root (cm), number of leaves and dry biomass. The data were subjected to analysis of variance (ANOVA) and means compared by Tukey’s test at p < 0.05. The survival and rooting percentages were higher than 70% in all treatments. There was no significance for the interaction of the factors analyzed, neither for the effect of AIB, analyzed in isolation. The type of stake exerted the greatest influence, with emphasis on the cuttings with leaves, which stood out in most of the factors analyzed. The propagation of H. sucuuba to cuttings was considered viable, and the maintenance of the leaves, associated with juvenility of the propagules, was determining factors in the conditions in which this study was developed.

Apocynaceae, rooting, auxins, indolbutyric acid, asexual propagation.

Article Details

How to Cite
Vasconcelos, R. G., Medeiros, R. da S., Silva, D. da, & Sampaio, P. de T. B. (2020). Vegetative Propagation of Himatanthus sucuuba (Spruce ex Müll. Arg.) Woodson by Stem Cuttings. Journal of Experimental Agriculture International, 42(2), 8-15.
Original Research Article


Lorenzi H. Brazilian Trees: A guide to the identification and cultivation of Brazilian native trees. 3th Ed. Portuguese. Nova Odessa, SP: Plantarum; 1998.

Revilla J. Amazonian Plants: economic and sustainable opportunities. 3th ed. Portuguese. Manaus, Amazonas: INPA/SEBRAE; 2001.

Rios MNS, Pastore Júnior F, editors. Amazonian Plants: 450 species for general use. 1th ed. Portuguese. Brasília, DF: University of Brasilia, Central Library; 2011.
(Accessed 01 September 2019)

Silva JRA, Rezende CM, Pinto AC, Amaral ACF. Cytotoxicity and antibacterial studies of iridoids and phenolic compounds isolated from the latex of Himatanthus sucuuba. African J. Biotechnol. 2010; 9(43):7357–7360.
(Accessed 25 September 2019)

Viljoen A, Mncwangi N, Vermaak I. Anti-Inflammatory Iridoids of botanical origin. Curr. Med. Chem. 2012;19(14):2104–2127.
(Accessed 05 September 2019)

Soares DC, Andrade AL, Delorenzi JC, Silva JR, Freire-de-Lima L, Falcão CA, et al. Leishmanicidal activity of Himatanthus sucuuba latex against Leishmania amazonensis. Parasitol. Int. 2010;59(2): 173–177.
(Accessed 15 September 2019)

Hartmann HT, Kester DE, Daves Jr FT, Geneve RL. Plant propagation: Principles and practices. 8th ed. Boston: Prentice-Hall; 2011.

Dias PC, Oliveira LS, Xavier A, Wendling I. Cutting and mini-cutting techniques of Brazil wood species. Pesqui. Florest. Bras. Portuguese. 2012;32(72):453–462.
(Accessed 02 October 2019)

Simão E, Nakamura AT, Takaki M. Harvest period and germination capacity of Tibouchina mutabilis (Vell.) Cogn. (Melastomataceae) seeds. Biota Neotrop. Portuguese. 2007;7(1):67–73.
(Accessed 24 October 2019)

Abiyu A, Dejene T, Eshete A, Sisay K. Vegetative propagation of Boswellia papyrifera: Time of collection and propagule size affect survival and establishment. J. Arid Environ. 2016;133: 122–124.
(Accessed 24 October 2019)

Taiz L, Zeiger E, Moller IM, Murphy A, editors. Plant physiology and development. Portuguese. 6th ed. Porto Alegre, RS: Artmed; 2017.

Neves TDS, Carpanezzi AA, Zuffellato-Ribas KC, Marenco RA. Rooting of Brazilian coral as a function of cutting types and season oh the year. Pesqui. Agropecuária Bras. Portuguese. 2006; 41(12):1699–1705.
(Accessed 14 October 2019)
Available: sci_abstract&tlng=pt

Moraes ER, Santos MS, Peixoto JVM, Golinski J. Production golden dew drop seedlings from different sizes of cuttings and amounts of leaves. Enciclopédia Biosf. Portuguese. 2016;3(23):1063–1072.
(Accessed 22 October 2019)

Santos JP, Davide AC, Teixeira LAF, Melo AJS, Melo LA. Rooting of lignified cuttings from forest species. Cerne. Portuguese. 2011;17(3):293–301.
(Accessed 02 September 2019)

Costa CT, Almeida MR, Ruedell CM, Schwambach J, Maraschin FS, Fett-Neto AG. When stress and development go hand in hand: Main hormonal controls of adventitious rooting in cuttings. Front. Plant Sci. 2013;4:1–19.
(Accessed 16 October 2019)

Tsafouros A, Frantzeskaki A, Assimakopoulou A, Roussos PA, “Spatial and temporal changes of mineral nutrients and carbohydrates in cuttings of four stone fruit rootstocks and their contribution to rooting potential. Sci. Hortic. (Amsterdam). 2019;253:227–240.
(Accessed 29 September 2019)

Barbosa PHD, Costa ACL, Cunha AC, Silva Junior JA. Weather elements of variability and thermal confort in different environments in the Brazilian Amazon. Rev. Bras. Climatol. Portuguese. 2015; 11(17): 98–118.
(Accessed 14 September 2019)

Souza KP. In vitro propagation of Himatanthus sucuuba Wood, a medicinal species from the Amazon. 57f. Portuguese. Thesis (Master in Bio-technology and Natural Resources in the Amazon), State University of Amazonas, Manaus; 2017.
(Accessed 02 September 2019)

Team RCR: A language and environment for statistical computing. R Foundation for Statistical Computing, Viena, Austria; 2016.

Souza AFF, Silva Neto EC, Coelho Júnior MG, Nogueira JKS, Cordeiro AAS. Effect of different concentrations of IBA on propagation by cuttings of Tabernaemontana divaricata (L.). Agrar. Acad. Portuguese. 2018;5(9):454–467.
(Accessed 18 September 2019)

Betanin L, Nienow AA. Vegetative propagation of the Brazilian coral (Erythrina falcata Benth.) by stem and foliar cutting. Semin. Agrar. Portuguese. 2010;31(4):871–880.
(Accessed 03 October 2019)

Leandro RC, Yuyama K. Rooting of cutia nut cuttings with indolbutiric acid. Acta Amaz. Portuguese. 2008;38(3):421– 430.
(Accessed 17 October 2019)

Oliveira MC, Ribeiro JF, Rios MNS, Rezende ME. Rooting of cuttings for the seedling production of native species of gallery forests. Technical recommendation, 41. Portuguese. Brasília: Emprapa Cerrados; 2001.
(Accessed 27 October 2019)

Xavier A, Wendling I, Silva RL. Clonal forestry: Principles and techniques. 2th ed. Portuguese. Viçosa, MG: UFV; 2013.

Stuepp CA, Zuffellato-Ribas KC, Wendling I, Koehler HS, Bona C. Leaf presence and indolbutiric acid on cuttings rooting of dragon free. Comun. Sci. Portuguese. 2015;6(2):181–193.
(Accessed 2 October 2019) Available:

Tognon GB, Petry C. Stem cutting of Ipomoea cairica (L.) Sweet. Rev. Bras. Plantas Med. Portuguese. 2012;14(3): 470–475.
(Accessed 04 November 2019)
Available: 300008

Vignolo GK, Picolotto L, Gonçalves MA, Pereira IDS, Antunes LEC. Presence of leaves on rooting of blackberry. Cienc. Rural. Portuguese. 2014;44(3):467–472.
(Accessed 10 November 2019)