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The effect of ethanolic extract of Spirulina platensis (EESP) on reproductive function of female guinea pigs exposed to oxidative stress induced by lead acetate was explored. 60 mature female guinea pigs were used. They were divided into 6 groups of 10 guinea pigs each. For 3 months, pigs in Groups 1 were orally given distilled water daily; Group 2 were treated with lead; Group 3: received lead acetate and Vitamin C while Groups 4,5 and 6 were given lead acetate with 50, 100 and 200 mg of EESP/kg.bw respectively by gavage. At the end of trial (90 days), 6 female pigs per group were sacrificed and some reproductive characteristics, oxidative stress and hepatic toxicity markers were evaluated. Results revealed that, exposure to Lead acetate induced a significant decrease (p < 0.05) of fertility index, reproductive organs and hormone concentrations. It equally led to a significant (p < 0.05) decline of serum content in superoxide dismutase, catalase and total peroxidase activities. The reverse effect was noted in malondialdehyde concentration, hepatic and renal toxicity biomarkers. Histological examination of the ovaries of lead treated females revealed lesions and severe degenerative changes in the structures of the ovaries. However, the administration of ethanolic extract of Spirulina platensis to treated females alleviated the toxicity of reproductive function and oxidative damage induced by lead acetate. In conclusion, the exposure of animals to lead acetate had negative effects on guinea pig reproductive characteristics. The co-exposition to lead acetate and ethanolic extract of Spirulina platensis however, reestablishes normal reproductive characteristic values.
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