Journal of Experimental Agriculture International

Potential Secondary Metabolites of Streptomyces sp. and Trichoderma sp. in Suppressing the Percentage of Spodoptera litura Attacks on Corn Plants

Journal of Experimental Agriculture International, Volume 44, Issue 12, Page 13-20
DOI: 10.9734/jeai/2022/v44i122073

Abstract


Spodoptera litura F. (Lepidoptera:Noctuidae) is one of the main pests on corn which is polyphagous and can cause crop failure due to damage to the leaves of the plant. Secondary metabolite compounds produced by microorganisms have many roles and functions, namely as compounds to protect plants from pest attacks. Streptomyces sp. and Trichoderma sp. are biological control agent which have the potential to produce chitinase enzymes capable of degrading the cell walls of larval and pupal stages. This study aims to determine the potential of secondary metabolites of Streptomyces sp. and Trichoderma sp. on various production media on the percentage of S. litura larval attack on corn plants. The study was conducted using a completely randomized factorial design with the first factor being the types and combination of biological control agents used, namely Streptomyces sp. and Trichoderma sp. While the second factor is the type of propagation media used i.e glucose nitrate (GN) and potato dextrose agar (PDA). The results showed that the combination of Streptomyces sp. and Trichoderma sp. on PDA production media with a concentration ratio of 5:1 can increase the potential of secondary metabolites in suppressing the percentage of S. litura larval attack on corn plants by up to 10%.

Keywords:
  • Production media
  • mortality
  • larval

How to Cite

Suryaminarsih, P., Nurfitriana, I., Mujoko, T., & Rahmadhini, N. (2022). Potential Secondary Metabolites of Streptomyces sp. and Trichoderma sp. in Suppressing the Percentage of Spodoptera litura Attacks on Corn Plants. Journal of Experimental Agriculture International, 44(12), 13–20. https://doi.org/10.9734/jeai/2022/v44i122073

References

Eppo. PM 7/124 (1) Spodoptera littoralis, Spodoptera litura, Spodoptera frugiperda, Spodoptera eridania. EPPO Bulletin. 2016; 410–44.

Fitriani UL, Ahdin Gassa DAN. The predation of Euborellia annulata (Dermaptera: Anisolabididae) and its preference in various larval instars of Spodoptera litura. Phytomedicine. 2011; 7(3):182 – 185.

Arif Adiba. The effect of chemicals on the use of environmental pesticides. Jf Fik Uinam. 2015;3(4):134–43.

Soesanto Loekas. Secondary metabolites of biological control agencies: A new breakthrough in control of plantation plant pest organisms. Purwokerto; 2015.

Kaur, Talwinder, Arti Vasudev, Satwinder Kaur Sohal, Rajesh Kumari Manhas. Insecticidal and growth inhibitory potential of streptomyces hydrogenans DH16 on major pest of India, Spodoptera litura (Fab.) (Lepidoptera: Noctuidae). BMC Microbiology. 2014;14(1):1–9.

Li S, Yang B, Tan GY, Ouyang LM, Qiu S, Wang W, Zhang L. Polyketide pesticides from actinomycetes. Current Opinion in Biotechnology. 2021;69:299-307.

Bapatla KG, Kumar K, Kumar L, Singh B, Singh NP. Anti-larval activity of actinobacterial extract for Helicoverpa armigera and Spodoptera litura. International Journal of Tropical Insect Science. 2022;42(1): 495-505.

Hoster, Frank, Jessica E. Schmitz, Rolf Daniel. Enrichment of chitinolytic microorganisms: isolation and characterization of a chitinase exhibiting antifungal activity against phytopathogenic fungi from a novel streptomyces strain. Applied Microbiology and Biotechnology; 2005.

Dicky Dharmawan, Penta Suryaminarsih, Wiwik Sri Harijani. Effectiveness of Streptomyces sp. and Trichoderma sp. as an entomopathogen against rhinoceros beetle larva (Oryctes rhinoceros L.). Plumules. 2018;1–9.

Fitriana IN, Suryaminarsih P, Mujoko T. Potential of Multientomopa Streptomyces sp. and Tripchoderma sp. in potato extract broth and glucose nitrate broth media on pests (Spodoptera litura) eating behavior by In vitro test. Nusantara Science and Technology Proceedings. 2018; 270-276.

Harijani, Wiwik Sri, Penta Suryaminarsih. Effect of several isolates of biological agents Actynomycetes spp. against insect morphology bactrocera sp. 2014;114–17 in Research Month V.

Fitriana IN, Penta Suryaminarsih, Wanti Mindari, Sri Wiyatiningsih. Multiantagonist growth study of Trichoderma sp. and Streptomyces sp. in root suspension, liquid humate and sugar potato extract. Agrotechnology Scientific Periodic– Plumula. 2020;7(1):25–32.

Hong ME, Lee KS, Yu BJ, Sung YJ, Park SM, Koo HM, Jin YS. Identification of gene targets eliciting improved alcohol tolerance in Saccharomyces cerevisiae through inverse metabolic engineering. Journal of Biotechnology. 2010;149(1-2):52-59.

Harni, Rita, Widi Amaria, Herliyati Mahsunah. Potential of secondary metabolites of Trichoderma spp. to control vascular streak dieback (Vsd) disease in potential cocoa seeds of Trichoderma spp. Secondary metabolite in controlling vascular streak dieback ( Vsd ) on cacao seedlings. Industrial Plants And Refreshments. 2017;57–66.

Nafisah, Hidayatun, Sri Pujiyanto, Budi Raharjo. Isolation and testing of chitinase activity of bacterial isolates from dieng geothermal area. Biome: Biological Scientific Periodic. 2017; 19(1):22.

Wibowo RH, Sipriyadi NR. Mubarik I. Rusmana, Suhartono MT. Isolation and screening of soil chitinolytic actinobacteria as the anti-fungal producer of plant pathogens. Elkawnie Journal of Science and Technologiy. 2020;6(2).

Mujoko Tri, Ika Rochdjatun Sastrahidayat, Tutung Hadiastono, Syamsuddin Djauhari. Antagonistic effect of Streptomyces spp. on spore germination and mycelial growth of Fusarium oxysporum f.Sp. Lycopersici. International Journal of Biosciences (IJB). 2014;5(9):414–22.

Krishanti NPRA, Wikantyoso B, Zulfitri A, Zulfiana D. Entomopathogenic bacteria as biocontrol agent against Spodoptera litura (F.) larval [entomopathogenic bacteria as biocontrol agent against Spodoptera litura (F.) Larval]. Biology News. 2017;16(1): 13–21.

Singh, Gursharan, Aditya Bhalla, Jasvinder Singh Bhatti, Sanjeev Chandel. Potential of chitinases as a biopesticide against agriculturally harmful fungi and insects. Journal Of Microbiology And Biotechnology. 2014;3(1):27–32.

Schünemann, Rogério, Neiva Knaak, Lidia Mariana Fiuza. Mode of action and specificity of bacillus thuringiensis toxins in the control of caterpillars and stink bugs in soybean culture. ISRN Microbiology. 2014;1–12.

Arasu, Mariadhas Valan, Naif Abdullah Al-Dhabi, Valsalam Saritha, Veeramuthu Duraipandiyan, Chinnasamy Mut Hukumar, Sun Ju Kim. Antifeedant, larvicidal and growth inhibitory bioactivities of novel polyketide metabolite isolated from Streptomyces sp. AP-123 against Helicoverpa armigera and Spodoptera litura. BMC Microbiology. 2013;13(1).

Subbanna ARNS, Rajasekhara H, Stanley J, Mishra KK, Pattanayak A. 2018. Pesticidal prospectives of chitinolytic bacteria in agricultural pest management. Soil Biology and Biochemistry. 2013;116: 52–66.

Ishaaya, Isaac, Svetlana Kontsedalov, A. Rami Horowitz. Emamectin, a novel insecticide for controlling field crop pests. Pest Management Science. 2002;58(11): 1091–95.

Safri, Muchamad, Penta Suryaminarsih Wiwik Sri Harijani. Test of pupa viability of fruit flies (Bactrocera sp.) to imago by giving Streptomyces sp. Biological Agent. 2016;5(1):39–49.

Contreras-Cornejo, Hexon Angel, Lourdes Macías-Rodríguez, Ek del-Val, John Larsen. The root endophytic fungus Trichoderma atroviride induces foliar herbivory resistance in maize plants. Applied Soil Ecology. 2018;124:45–53.

  • 114 times
    PDF Download: 57 times

Download Statistics

Make a Submission

Information

Current Issue