Potential Secondary Metabolites of Streptomyces sp. and Trichoderma sp. in Suppressing the Percentage of Spodoptera litura Attacks on Corn Plants
Penta Suryaminarsih *
UPN “Veteran” Jawa Timur, Jalan Tamtama 27, Surabaya-64242, Indonesia.
Ika Nurfitriana
UPN “Veteran” Jawa Timur, Jalan Medayu Selatan XXI blok u/18, Surabaya-60294, Indonesia.
Tri Mujoko
UPN “Veteran” Jawa Timur, Jl. Batu Jajar I/29, Kab, Malang-65133, Indonesia.
Noni Rahmadhini
UPN “Veteran” Jawa Timur, Perumahan Pelem Indah Blok D-7, Pelem, Pare, Kediri-64213, Indonesia.
*Author to whom correspondence should be addressed.
Abstract
Spodoptera litura F. (Lepidoptera:Noctuidae) is one of the main pests on corn which is polyphagous and can cause crop failure due to damage to the leaves of the plant. Secondary metabolite compounds produced by microorganisms have many roles and functions, namely as compounds to protect plants from pest attacks. Streptomyces sp. and Trichoderma sp. are biological control agent which have the potential to produce chitinase enzymes capable of degrading the cell walls of larval and pupal stages. This study aims to determine the potential of secondary metabolites of Streptomyces sp. and Trichoderma sp. on various production media on the percentage of S. litura larval attack on corn plants. The study was conducted using a completely randomized factorial design with the first factor being the types and combination of biological control agents used, namely Streptomyces sp. and Trichoderma sp. While the second factor is the type of propagation media used i.e glucose nitrate (GN) and potato dextrose agar (PDA). The results showed that the combination of Streptomyces sp. and Trichoderma sp. on PDA production media with a concentration ratio of 5:1 can increase the potential of secondary metabolites in suppressing the percentage of S. litura larval attack on corn plants by up to 10%.
Keywords: Production media, mortality, larval