Appraising the Biochemical Responses in Wheat (Triticum aestivum L.) Seedlings under Various Seed Treatments
Sultan Singh *
Department of Seed Science and Technology, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004, India.
Virender Singh Mor
Department of Seed Science and Technology, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004, India.
Axay Bhuker
Department of Seed Science and Technology, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004, India.
Digamber
Department of Seed Science and Technology, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004, India.
Rahul Kumar
Department of Seed Science and Technology, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004, India.
Gagandeep Singh
Department of Seed Science and Technology, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004, India.
*Author to whom correspondence should be addressed.
Abstract
A study was conducted to analyse the effect of pesticides and biofertilizers on enzymatic activity of dehydrogenase, catalase, peroxidase and superoxide dismutase (SOD) in seedlings of wheat (Triticum aestivum L.) varieties (WH1105 and WH1124) and their old and fresh seed lots. This experiment was conducted in the laboratories at the Department of Seed Science and Technology, CCS Haryana Agricultural University in Hisar during 2020. A total of 16 treatment combinations were applied to the seeds in the experiment, including control, which were subsequently used for germination. The seedlings of the wheat cultivars were germinated in between paper at controlled temperature 20oC. 4 days old germinated seedlings were used for the analyses of the biochemical parameters. This finding revealed that the seedlings germinated from seeds treated with T5-Azotobacter+PSB exhibited highest biochemical activity, followed by T4-Phosphate Solubilizing Bacteria. The application of biofertilizers enhanced the dehydrogenase, catalase, peroxidase and superoxide dismutase enzyme activities, whereas the T1-Chlorpyrifos reduced the biochemical activities of the wheat seedlings. The results demonstrated that Chlorpyrifos had an adverse effect on the biochemical activities and inhibiting seedlings optimal growth and inducing stress-related challenges for wheat seedlings.
Keywords: Biochemical, catalase, dehydrogenase, enzyme activity, peroxidase, superoxide dismutase, wheat