Suppression of Sugarcane Red Rot Disease through Its Rhizospheric Mycoflora

Priyam Vandana *

Division of Crop Protection, ICAR-Indian Institute of Sugarcane Research, Lucknow, India and Department of Molecular and Cellular Engineering, Sam Higginbottom University of Agriculture Technology and Sciences, Allahabad, India.

Dinesh Singh

Division of Crop Protection, ICAR-Indian Institute of Sugarcane Research, Lucknow, India.

Sangeeta Srivastava

Division of Crop Improvement, ICAR-Indian Institute of Sugarcane Research, Lucknow, India.

Gurudayal Ram Guru

Department of Molecular and Cellular Engineering, Sam Higginbottom University of Agriculture Technology and Sciences, Allahabad, India.

*Author to whom correspondence should be addressed.


Red rot disease of sugarcane is so devastating in nature that it has been referred to as “cancer of sugarcane”. The sugar industry in India suffered losses more than 500 million US dollars every year due to red rot. So, there is a need to look over the management and control of the disease. Biological control method is an alternative method of chemical pesticides which are an innovative, cost effective and eco-friendly approach. Soil samples were collected from sugarcane rhizosphere of four different varieties viz., CoPk 05191 and CoLk 94184 (resistant to red rot) and CoJ 64 and Co 1148 (susceptible to red rot) planted at ICAR-Indian Institute of Sugarcane Research, Lucknow experimental farm. The samples were subjected for the isolation of rhizospheric mycoflora on Potato Dextrose Agar media and antagonistic activities of the isolates were tested against red rot pathogens (Cf 07, Cf 08 and Cf 09). Isolates showing more than 50 % inhibition with all the three pathotypes (Cf 07, Cf 08 and Cf 09) were selected for further study. Among the twelve selected isolates used for field experiment for the management of red rot, isolate T16 was found to be highly effective. The suppression of red rot offered by rhizospheric isolates is probably due to induced systemic resistance in sugarcane plants or by the enzymatic action of metabolites produced by the rhizospheric mycoflora. The different levels of red rot infected seed cane viz., 5%, 10%, 15%, 20%, 25%, 30%, 35% and 40% were treated with MHAT and planted along with healthy seed canes, infected seed canes, as a control to work out the losses caused by different level of red rot seed infection. The result revealed that a yield loss was recorded with the infected seed. The losses were directly proportionate with the level of red rot infected seed.

Keywords: Sugarcane, rhizospheric mycoflora, Colletotrichum falcatum, antagonistic activity

How to Cite

Vandana , P., Singh, D., Srivastava , S., & Guru , G. R. (2024). Suppression of Sugarcane Red Rot Disease through Its Rhizospheric Mycoflora. Journal of Experimental Agriculture International, 46(5), 98–109.


Download data is not yet available.


Shair F, Yasmin, H, Hassan MN, Alzahrani OM, Noureldeen A. Pseudomonas spp. Mediate defense response in sugarcane through differential exudation of root phenolics. Saudi Journal of Biological Sciences. 2021;28:7528–7538.

Yadav SK, Yadav S. Doubling the income of sugarcane farmers with reduced cost in India and cane scenario in major sugarcane growing countries. International Journal of Advanced Research.2017;5(8):1586-1592.

Rott P, Davis MJ. Leaf scald. In: A Guide to Sugarcane Diseases (eds P. Rott, R. A., Bailey, J. C., Comstock, B. J., Croft and A. S. Saumtally), CIRAD and International Society of Sugarcane Technologists, Montpelllier. 2000;38-44.

Bharti YP, Vishwakarma SK, Kumar A, Singh A, Sharma ML, Shukla DN. Physiological and pathological aspects of some new isolates of Colletotrichum falcatum causing red rot disease in Saccharumspp Complex. Acta Phytopathologicaet Entomologica Hungarica.2012; 47(1): 35-50.

Jayashree J, Selvi A, Nair NV. Characterization of resistance gene analog polymorphism in sugarcane cultivars with varying levels of red rot resistance. Electronic Journal of Plant Breeding. 2010;1(4):1191-1199.

Viswanathan R. Red rot of sugarcane (Colletotrichum falcatum Went). CAB Reviews. 2021; (023):1-57. Available: Available:

Gowsalya A, Ponnusami V, Sugumaran KR. Isolation of bacteria from soil sample for exo-polysaccharide production, International Journal of Chem. Tech. Research. 2014;6:2925-2928.

Juma EOA, Musyimi DM, George O. Enumeration and identification of rhizospheric microorganisms of sugarcane variety Co 421 in Kibos Kisumu county Kenya. Journal of Asian Scientific Research. 2018;8(3):113-127.

Harman GE. Overview of mechanism and uses of Trichoderma spp. Phytopathology.2006;96:190-194.

Singh V, Srivastava SN, Lal RJ, Awasthi SK, Joshi BB. Biological control of red rot disease of sugarcane through Trichoderma harzianum and Trichoderma viride. Indian Phytopath. 2008;61(4):486-491.

Yadav RL, Yadav DV, Sharma AK, and Singh GK. Envisioning Improved sugarcane production technology for high yields and high sugar recovery in India. Sugar Tech. 2009;11(1):1-11.

Goh TK. Single spore isolation using a handmade glass needle, Fungal Divers. 1999;2:47-63

Baijal U, Mehrotra BS. The genus Cunninghamella-are assessment. Sydowia.1980;33:1-13.

Bisset J. A revision of the genus Trichoderma II. Infra generic classification. Can. J. Bot. 1991a;69:2357-72.

Bisset J. A revision of the genus Trichoderma III. Section Pachybasium. Can. J. Bot. 1991b;69:2373-417.

Gaddeyya G, Niharika PS, Bharathi P, Kumar PKR. Isolation and identification of soil mycoflora in different crop fields at Salur Mandal. Advanced Applied Sciences Research. 2012;3:2020-2026.

Sumana A, Singh P. Evaluation of epiphytic microflora as antagonists of red rot pathogen, Colletotrichum falcatum in sugarcane under subtropical conditions. Journal of Biological Control. 2012;26(4): 351-360.

Yasmin H, Naz R, Nosheen A, Hassan MN, Ilyas N, Sajjad M, Anjum S, Gao X, Geng Z. Identification of new biocontrol agent against charcoal rot disease caused by Macrophomina phaseolina in soybean (Glycine max L.) Sustainability. 2020; 12(17):6856. Available:

Lunge AG, Patil AS. Characterization of efficient chitinolytic enzyme producing Trichoderma species: a tool for better antagonistic approach. International Journal of Science, Environment and Technology. 2012;1(5):377-385.

Samuels GJ, Dodd SL, Gams W, Castlebury LA, Petrini O. Trichoderma species associated with the green mold epidemic of commercially grown Agaricu sbisporus. Mycologia.2002;94:146-170.

Boureghda H, Bouznad Z, Decock C. Cultural and molecular characterizations of some isolates of Trichoderma spp. Arab Journal of Plant Protection. 2008;26:75-80.

Joshi D, Misra SC. Characterization of Trichoderma isolates from sugarcane agro-ecosystem and their efficacy against Colletotrichum falcatum causing red rot of sugarcane. Sugar Tech.2013;15(2):192-196.

Samuels GJ. Trichoderma: systematics, the sexual state, andecology. Phytopathology.2006;96:195-206.

Haque MM, Haque MA, Ilias G, and Molla AH. Trichoderma- Enriched Biofertilizer: A Prospective Substitute of Inorganic Fertilizer for Mustard (Brassica campestris) Production. Agric.1970;8(2):66-73.

Blaszczyk L, Siwulski M, Sobieralski K, Lisiecka J, Jędryczka M. Trichoderma spp. Application and prospects for use in organic farming and industry. J. Plant Prot. Res. 2014;54(4):309-317.

Benitez T, Rincon AM, Limon MC, Codon AC. Biocontrol mechanisms of Trichoderma strains. Int. Microbiol. 2004;7(4):249-260.

Patel P, Shah R, Joshi B, Ramar K, Natarajan A. Molecular identification and biocontrol activity of sugarcane rhizosphere bacteria against red rot pathogen Colletotrichum falcatum. Biotechnology Reports. 2019;3:e00317. Available:

Varma PK, Kumar VKK, Suresh M, Chandrasekhar V, Bharathalakshmi M, Jamuna P. Bacillus amyloliquefaciens (RB19): A potential PGPR in managing sugarcane red rot disease. Journal of Pharmacognosy and Phytochemistry. 2019;8(6):2255-2261.

Aslam S, Hamid MI, Ghazanfar MU, Akhtar N. Assessment of sugarcane genotypes for red rot resistance and antifungal activity of rhizosphere microbiota against Colletotricum falcatum. Intl JAgric Biol. 2021;26:241‒250

Zia MA, Yasmin H, Shair F, Jabeen Z, Mumtaz S, Hayat Z, Shah, SZH. Afghan S, Hafeez FY, Hassan MN. Glucanolytic rhizobacteria produce antifungal metabolites and elicit ROS scavenging system in sugarcane. Sugar Tech. 2019; 21(2):244-255. Available:

Viswanathan R, P Malathi. Biocontrol strategies to manage fungal diseases in Sugarcane. Sugar Technol.2019;21:202‒212.

Amna Y, Xia Y, Farooq MA, Javed MT, Kamran MA, Mukhtar T. Multi-stress tolerant PGPR Bacillus xiamenensis pm14 activating sugarcane (Saccharum officinarum L.) red rot disease resistance. Plant Physiol. Biochem. 2020;151:640–649. DOI: 10.1016/j.plaphy.2020.04.016

Xie L, Liu L, Luo Y, Rao X, Di Y, Liu H, Qian Z, Shen Q, He L, Li F. Complete genome sequence of biocontrol strain Bacillus velezensis YC89 and its biocontrol potential against sugarcane red rot. Front. Microbiol. 2023; 14: 1180474. DOI: 10.3389/fmicb.2023.1180474

Zin NA, Badaluddin NA. Biological functions of Trichoderma spp. for agriculture applications. Annals of Agricultural Sciences. 2020;65:168–178.

Howell CR, Hanson LE, Stipanovic RD, Puckhaber LS. Induction of Terpenoid Synthesis in Cotton Roots and Control of Rhizoctoniasolani by Seed Treatment with Trichoderma virens. Phytopathology.2000;90:248-252.

Joshi D, Singh P, Singh AK, Lal RJ, Tripathi N. Antifungal potential of metabolites from Trichoderma sp. against Colletotrichum falcatum Went causing red rot of Sugarcane. Sugar Tech.2016;18:529-536.

Joshi D, Singh P, Holkar SK, Kumar S. Trichoderma-mediated suppression of red rot of sugarcane under field conditions in subtropical India. Sugar Tech; 2018. DOI: 10.1007/s12355-018-0624-0.

Singh V, Joshi BB, Awasthi SK, Srivastava SN. Ecofriendly management of red rot disease of sugarcane with Trichoderma strains. Sugar Tech. 2008a;10:158-161.

Singh V, Lal RJ, Awasthi SK, Verma MR. Managing red rot of sugarcane by Trichoderma harzianum. Indian Sugar. 2009;59:25-30.

Freeman S, Miz D, Kolesnik I, Barbul O, Zveibil A, Maymon M, Nitzani Y, Krihshner B, Rav-David D, Bilu A, Dag A, Shafir S, Elad Y. Trichodermabiocontrol of Colletotrichum acutatum and Botrytis cinerea and survival in strawberry. European Journal of Plant Pathology. 2004;110:361-370.

Sanchez V, Rebolledo O, Picaso RM, Cardenas E, Cordova J, Gonzalez O, Samuels GJ. In vitro antagonism of Thielaviopsisparadoxa by Trichoderma longibrachiatum. Mycopathologia. 2007; 163:49-58.

Hossain MI, Ahmad K, Siddiqui Y, Saad N, Rahman Z, Haruna AO, Bejo SK. Current and prospective strategies on detecting and managing Colletotrichum falcatumcausing red rot of sugarcane. Agronomy. 2020;10:1253. DOI:10.3390/agronomy10091253

Talukder MI, Kamal MM, Iqbal M, Rahman S. Bangladesh Akher Rog Balai Somuho O Tar Pratikar; Bangladesh Sugarcane Research Institute: Ishurdi, Pabna, Bangladesh. 2010;5–10.