Evaluation of Antimicrobial Activity of Neem Cake and Bio-Agents on Early Blight (Alternaria solani) of Tomato (Lycopersicon esculentum L.)

Thadaka Devagoud *

Department of Plant Pathology, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, Uttar Pradesh, India.

Sobita Simon

Department of Plant Pathology, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, Uttar Pradesh, India.

*Author to whom correspondence should be addressed.


Early blight is the important disease of tomato because it causes huge economic loss to the farmer every year. Alternaria solani cause early blight of tomato and it is considered weed of field because of its wide adaptability under different environment. Use of fungicides for the management of disease in crop puts a large number of negative health and environmental effects therefore, the urgent need for a more sustainable and ecological approach to manage disease without fungicides. To avoid relying solely on chemicals and to identify a viable alternative component,this experiment was conducted to conclude the Evaluation of antimicrobial activity of Neem cake and bio-agents on early blight (Alternaria solani) of tomato (Lycopersicon esculentum L.) by soil application with Neem cake @ 250kg/ha and seedling treatment with bio agents in single and combination viz Pseudomonas fluorescens @10g/lit, Bacillu thuringiensis @10g/lit, Trichoderma viride @10g/lit, Pseudomonas fluorescens@5g/lit +Bacillus thuringiensis @5g/lit, Pseudomonas fluorescens@5g/lit + Trichoderma viride @5g/lit and Trichoderma viride @ 5g/lit + Bacillus thuringiensis @5g/lit both in-vivo and in-vitro for their effectiveness to manage early blight of tomato caused by Alternaria solani .All the treatments were found significantly reduced the severity of the disease and increased growth parameters, Among all the treatments Neem cake@250kg/ha+ Pseudomonas fluorescens @5g/lit + Trichoderma viride @ 5g/lit followed by Neem cake@250kg/ha + Trichoderma viride @5g/lit + Bacillus thuringiensis @5g/lit were significantly superior over other treatments in reducing early blight infection and also in increasing growth parameters of the crop. For in-vitro studies only bio agents were selected among the treatments Pseudomonas fluorescens + Trichoderma viride showed highest mycelia inhibition (89.3%) followed by Trichoderma viride + Bacillus thuringiensis (86.6%). The highest cost benefit ratio was obtained with Neem cake 250kg/ha + Pseudomonas fluorescens @5g/lit + Trichoderma viride @5g/lit treatment (1:3.85). while other treatments also showed significantly effective for the checking of disease intensity and yield over control in the field condition.

Keywords: Alternaria solani, tomato, soil application, seedling treatment, neem cake, bio-agents, In- vivo, In-vitro

How to Cite

Devagoud, T., & Simon , S. (2024). Evaluation of Antimicrobial Activity of Neem Cake and Bio-Agents on Early Blight (Alternaria solani) of Tomato (Lycopersicon esculentum L.). Journal of Experimental Agriculture International, 46(5), 173–180. https://doi.org/10.9734/jeai/2024/v46i52368


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Agarwal S, Rao AV. Tomato lycopene and its role in human health and chronic diseases. 2000;163(6):739 - 44.

FAOSTAT. FAO statistics Database. Food and Agriculture organization of the United Nations; 2001.

Agrios GN. Plant Pathology. 5th edition, Elsevier Academic Press, New York; 2005.

Annonymous. Agricultural statistics at a glance 2021. Ministry of Agriculture and Farmer Welfare, Directorate of Economics and Statistics, New Delhi, India. 2005;235.

Basamma RH, Kulkarni S. Bio efficacy of Bacillus stubilis Against Foliar Fungal Diseases Of Tomato. International Journal of Applied and Pure Science and Agriculture. 2016;2(2).

Sharma RB, Zacharia S, Thakur MB. Management of early blight (Alternaria solani) in tomato by bio-agent, plant extract and fungicides. The Pharma Innovation Journal. 2022;11(7):1376-1379.

Jones JB, Jones JP, Stall RE. Zitter, TA. Compendium of Tomato Disease.American Phytopathological Society. 1991;73

Compant S, Reiter B, Sessitsch A, Nowak J, Clément C, Barka EA. Endophytic colonization of Vitis vinifera L. by plant growth-promoting bacterium Burkholderia sp. strain Ps JN; Applied Environmental Microbiology. 2005;71:1685–1693.

Datar VV, Mayee CD. Assessment of loss in tomato yield due to early blight, Indian Phytopath. 1981;34:191-195.

Ganeshan G, Kumar M. Pseudomonas fluorescens, a potential bacterial antagonist to control plant diseases. Journal of Plant Interactions. 2006;1(3):123-134.

Sherf AF, MacNab AA. Vegetable Diseases and Their Control. 1986;728

Peralta IE., Knapp S, Spooner DM. New species of wild tomatoes (Solanum section Lycopersicon: Solanaceae) from northern Peru. Systematic Botany. 2005;30:424–434.

Simmons EG. Alternaria themes and variations Species on Solanaceae. Mycotaxon. 2000;75:1-115:244- 286

Seyoun RK., Zacharia S, Bawane AS. Eco-Friendly management of alternaria leaf spot of brinjal (Solanum melongena L.). International Journal of Current Microbiology and Applied Sciences. 2021; 10(07):20-29.

Kakraliya SS, Choskit D, Pandit, D, Abrol, S. Effect of Bio-Agents, Neem Leaf Extract and Fungicides A gainst Alternaria Leaf Blight of Wheat (Triticum aestivum L.). Natural Products Chemistry & Research. 2017;5:8.

Krishnaraj KR, Murali S, Arunpandian S, Jayaraman K. Effect of soil application of neem cake amended with Trichoderma and Pseudomonas fluorescens on soil health and yield of tomato crop. International Journal of Applied and Pure Science and Agriculture. 2018;04(7):2394-5532.

Lokanadhan S, Muthukrishnan P, Jeyaraman, S. Neem products and their agricultural applications, Agricultural applications of neem products. Journal of Biopesticides. 2012;5:72-76.

Ramamoorthy V, Viswanathan R, Raguchander T, Prakasam V, Samiyappan R. Induction of systemic resistance by plant growth promoting rhizo bacteria in crop plants against pests and diseases. Crop Protection. 2001;20: 1– 11.

Suleiman SA, Simon S, Babychan M. Effect of Bioagents and their Consortia in the Management of Early Blight Disease of Potato. International Journal of Agriculture Innovations and Research. 2016;5(3)23 :19-1473.

Vincent JM. Distribution of fungal hyphae in the presence of certain inhibitors. Nature. 1927;159(4051):850.

Tran, N. Ha. (2010). Using Trichoderma species for biological control of plant pathogens in vietnam. Journal of International Society for Southeast Asian Agricultural Sciences. 2010;16(1):17-21.

Wheeler BEJ. An Introduction of Plant Diseases. John Willey and Sons Ltd. London. 1969;301.

Abdullahi S, Simon S, Babychan M. Effect of bioagents and their consortia in the management of early blight disease of potato. Int J Agri Res Innov Technol. 2016;5(3):495-8.