Plant Growth Regulators and Rooting Media: A Viable Approach for Growth and Performance of Citrus

Pritam Guchhait

Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara, India.

Suraj Varma *

Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara, India.

Dhrubajyoti Banerjee

Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara, India.

Sandeep Kumar

Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara, India.

Rajdip Halder

Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara, India.

Ankit Dahiya

Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara, India.

*Author to whom correspondence should be addressed.


Plant growth regulators accompanied with appropriate growth medium can boost up the growth and performance of plants to a greater extent. PGRs such as auxin, gibberellin and cytokine play an exaggerative role in growth and development of the citrus. Moreover, the study reflects a clear overview of the impact of PGRs and different growth media to encourage rooting percentage, number of leaves, survival percentage, sprouting time, and root length of citrus cuttings. Citrus is a versatile fruit crop having a huge number of varieties, cultivars, and species; comes in different colours and flavours. Although, there are different methods used in the multiplication of citrus plant which includes budding, grafting, cutting, air layering, etc. However, propagation by cutting technique is the most commercial and farmer’s friendly approach. Different elements such as concentration of PGRs, age of plants and environmental factors are responsible for the successful growth of the citrus cuttings. Naphthalene acetic acid (NAA) and indole-3-butyric acid (IBA) are the two types of auxin, which are most commonly utilized in citrus propagation. Rooting medium plays a crucial role in the supply the minerals and availability of water in citrus. An absolute rooting medium must have more enough nutrients and water holding capacity with better drainage as well as sufficient air spores for excellent gaseous exchange that fruitfully influence the rooting ability and also have a profound effect on growth and development in citrus cutting. The best time for cutting of citrus stems is in between the July and August.

Keywords: Plant growth regulators, growing medium, citrus cutting

How to Cite

Guchhait, P., Varma, S., Banerjee, D., Kumar, S., Halder, R., & Dahiya, A. (2024). Plant Growth Regulators and Rooting Media: A Viable Approach for Growth and Performance of Citrus. Journal of Experimental Agriculture International, 46(5), 366–378.


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Albrecht U, Bodaghi S, Meyering B, Bowman KD. Influence of rootstock propagation method on traits of grafted sweet orange trees. HortScience. 2020;55(5):729-737.

Al-Jabbari KH, Pakyürek M, Yavic A. Comparison of Rooting Situations for Salakhani and Zivzik Pomegranates under Different IBA Doses. Applied Ecology & Environmental Research. 2020; 18(1).

Al-Jubori MT, Al-Amery LKJ. Effect of Cytokinins BA, Kin, Auxin iaa, AND IBA on Propagating (Citrus Aurantiifolia) in Vitro. Int. J. Agricult. Stat. Sci. 2022;18(1):2033-2040.

Al-Zuhairi FF, Al-Aareji JM, Al-Taae AK. Effect of rootstock and bio-fertilizers on some mineral concentrations in the leaves of Local Lemon (Citrus limon L.) transplants and available nutrients in the Media. In IOP Conference Series: Earth and Environmental Science. 2021; 735(1):012049.

Anitha J, Kumar TS, Girwani A, Goud CR, Harshitha DN. Effect of foliar application of plant growth regulators and micronutrients on quality of acid lime (Citrus aurantifolia Swingle) Cv. Balaji; 2023.

Arunadevi A, Rajangam J, Venkatesan K. Effect of plant growth regulators on growth, yield, and quality of acid lime (Citrus aurantifolia Swingle.) var. PKM 1. Journal of Pharmacognosy and Phytochemistry. 2019;8(3):3438-3441

Brar HS, Thakur A, Singh H, Kaur N. Photoselective coverings influence plant growth, root development, and biddability of citrus plants in protected nurseries. Acta physiologiae plantarum. 2020;42:1-15.

Brischke C, Wegener FL. Impact of water holding capacity and moisture content of soil substrates on the moisture content of wood in terrestrial microcosms. Forests. 2019;10(6):485.

Budiarto R, Poerwanto R, Santosa E, Efendi D. A review of root pruning to regulate citrus growth. Journal of Tropical Crop Science. 2019;6(1):1-7.

Cao D, Chabikwa T, Barbier F, Dun EA, Fichtner F, Dong L, Kerr SC, Beveridge CA. Auxin-independent effects of apical dominance induce changes in phytohormones correlated with bud outgrowth. Plant Physiology. 2023;192(2):1420-1434.

Choudhary B, Sharma TR, Pandey SK, Paradkar VK. Effect of rootstock and growing media on seedling growth and success of micro budding technique of Mandarin (Citrus reticulata Blanco); 2022.

Das P, Prasad VM, Bahadur V, Topno SE, Paruchuru VK. Effect of Foliar Application of bio-regulators (GA3 and NAA) on Vegetative Growth Characters and Establishment of Lemon Citrus Limon (L.) cv. Eureka under Subtropical Prayagraj Agro Climatic Condition. In Biological Forum–An International Journal. 2021;13(3):159-162.

Deshlehra R, Pyasi R, Singh KV. Impact of growth regulators and micronutrients on growth, yield, and quality of acid lime (Citrus aurantifolia Swingle) under HDP system. The Pharm Innov J. 2022;11(2):362-6.

Dhakad A, Sonkar P, Bepari A, Kumar U. Effect of pre-harvest application of plant growth regulators and calcium salts on biochemical and shelf life of acid lime (Citrus aurantifolia Swingle). Journal of Pharmacognosy and Phytochemistry. 2020;9(4):1983-1985.

Eisa EA, Tilly-Mándy A, Honfi P, Shala AY, Gururani MA. Chrysanthemum: A comprehensive review of recent developments on in vitro regeneration. Biology. 2022;11(12):1774.

EL Sabagh A, Islam MS, Hossain A, Iqbal MA, Mubeen M, Waleed M, Reginato M, Battaglia M, Ahmed S, Rehman A, Arif M. Phytohormones as growth regulators during abiotic stress tolerance in plants. Frontiers in Agronomy. 2022;4:765068.

Esringü A, Ekinci M, Turan M. Effects of Different Growing Media on Growth Parameters of Zinnia (Zinnia elegans). Yuzuncu Yıl University Journal of Agricultural Sciences. 2022;32(1): 175-185.

Estrella-Maldonado H, Solís JRM, Rodríguez-Quibrera CG. Disinfection procedure for stem cuttings and in vitro production of axillary buds for Persian lime sanitation; 2022.

Ferlito F, Torrisi B, Allegra M, Stagno F, Caruso P, Fascella G. Evaluation of conifer wood biochar as growing media component for citrus nursery. Applied Sciences. 2020;10(5):1618.

Fruit D. Generation of Disease-Free Propagules of Dragon Fruit S. Vinodh*, Dipika Sarmah 2. Quality Transplant Production of Fruit Crops. 2023;109.

Gautam P, Tripathi SK, Kumar A, Prakash S, Sengar RS, Awasthi M, Maurya U, Kumar A, Effect of different Concentrations of PGRs on Shooting and Survival of Stem Cuttings in Lemon (Citrus limon Burm.) cv. Pant lemon-1, under Western UP conditions; 2022.

Ghimire BK, Kim SH, Yu CY, Chung IM. Biochemical and physiological changes during early adventitious root formation in chrysanthemum indicum linné cuttings. Plants. 2022;11(11):1440.

Gnawali P, Gurung M, Kadel S, Bhantana S, Chand NB, Pathak R, Poudel PR. Vegetative Propagation of Eureka Seedless Lemon (Citrus limon L. Cv Eureka Seedless) Using Different Types of Stem Cutting and Concentrations of Indole-3-Butyric Acid in Winter. Nepalese Horticulture. 2022;16(1):81-90.

Harshita GT, Singh G. The use of auxin and media for rooting of citrus stem cuttings: A review; 2022.

Hayat A, Asim M, Ashraf T, Haque E, Zulifqar R, Nasir M, Raza A, Khadija F, Afzal S, Ali S, Impact of different potting media on the growth of rough lemon (Citrus Jambhiri Lush). Journal of Innovative Sciences. 2023;9(1):154-162.

Idol T, Youkhana A, Santiago RP. Vegetative and micropropagation of Leucaena. Tropical Grasslands-Forrajes Tropicales. 2019;7(2):87-95

Kangabam L, Thokchom R, Naoroibam SD, Nigthoujam SD. Plant bioregulators and their effect on growth and development of Kachai lemon (Citrus jambhiri Lush.) in vitro. Pharm. J. 2020; 9(11):18-21.

Kavinprashanth R, Paramaguru P, Rani A, Sujatha KB. Impact of foliar application of growth regulators and micronutrients on yield and quality of acid lime (Citrus aurantifolia Swingle). Journal of Pharmacognosy and Phytochemistry. 2021;10(1):2091-2093.

Khan MN, Nabi G. Role of Auxin in vegetative growth, flowering, yield and fruit quality of Horticultural crops-A review. Pure and Applied Biology (PAB). 2023;12(2):1234-1241.

Khandaker MM, Saidi A, Badaluddin NA, Yusoff N, Majrashi A, Alenazi MM, Saifuddin M, Alam MA, Mohd KS. Effects of Indole-3-Butyric Acid (IBA) and rooting media on rooting and survival of air layered wax apple (Syzygium samarangense) CV Jambu Madu. Brazilian Journal of Biology. 2022;82:e256277.

Kour H, Beniwal B, Chhabra A, Pathlan N. Response of IBA on rooting behavior of Rough lemon (Citrus× Gambhir Lush) under various growing conditions; 2022.

Kumar A, Singh V, Johar V, Kumar P, Kadlag SK. Effect and Importance of plant growth regulators and molecular approaches on Fruit cuttings for the development of fruit crops: A.; 2023.

Kumar P, Singh V, Johar V, Kumar A, Kadlag SS. Uses of plant growth regulators and biofertilizers in fruit crops: A Review. International Journal of Environment and Climate Change. 2022;12(11):314-326.

Kumar R, Kumar A. Effect of indole-3-butyric acid concentrations and rooting media on growth and survival of stem cutting of lemon (Citrus limon Burm) Cv. pant lemon-1 under net house condition; 2022.

Kumar R, Singh JP. Influence of IBA and PHB on regeneration of Kagzi lime (Citrus aurantifolia Swingle) through stem cutting. IJCS. 2020;8(1):1952-1958.

Kumar S, Prasad VM, Bahadur V. Effects of Plant Growth Regulators (IBA) and Soil Media on Success, Growth, and Survival of Stem Cutting of Assam Lemon (Citrus lemon (L) Burm). International Journal of Plant & Soil Science. 2022;34(23):288-298.

Kumar V, Thakur D, Srivastava RP, Kaur DP, Singh KK. Effect of different concentrations of IBA and types of stem cuttings on the rooting in sweet orange (Citrus sinensis L. Osbeck); 2023.

Lal M. Effect of growing media on fruit crops: A review; 2023.

Magray MM. Plant Growth Regulators and Their Role in Horticultural Crop Production and Development. Research Management in Horticultural Crops. 2021;31.

Malakar A, Prakasha DP, Kulapati H, Reddi SG, Gollagi SG, Anand N, Satheesh P. Effect of growing media and plant growth regulators on rooting of different types of stem cuttings in acid-lime cv. Kagzi. International Journal of Current Microbiology and Applied Sciences. 2019; 8(10):2589-2605.

Mariano IA. Rooting Response of Selected Citrus SP. to Application of Different Hormones. EPRA International Journal of Agriculture and Rural Economic Research (ARER). 2022;10(6): pp.60-72.

Maurya P, Mukhim C, Prasad K, Majaw T, Kumar U, Agnihotri R, Kumar K. Influence of season, Indole 3-butyric acid and media on rooting and success of single leaf-bud cutting of lemon (Citrus limon Burm.) in Bihar. Scientist. 2022;1(3):459-469.

Patel SK, Singh JP, Yadav AK. Effect of IBA and NAA levels on growth traits of Kagzi lime (Citrus aurantifolia Swingle). Journal of Pharmacognosy and Phytochemistry. 2021;10(2): pp.892-897.

Priya VS, Kumar CSR, Poorniammal R, Rajangam J, Venkatesan K. Standardization of Propagation Technique and Media Combination in Acid Lime (Citrus aurantifolia) var. PKM. Int. J. Curr. Microbiol. App. Sci. 2020;9(10):2174-2184.

Qadri R, Hussain S, Akram MT, Khan MA, Khan MM, Hussain K, Khatana MA, Nadeem S, Khan UA. Impact of Different Growing Media and Gibberellic Acid (GA3) Concentrations on Rough Lemon (Citrus Jambhiri) Seed Germination and Its Growth Attributes. International Journal of Modern Agriculture. 2021;10(2):4471-4482.

Rajangam J, Sankar C, Kavino M. Effect of IBA on rooting of Acid lime (Citrus aurantifolia Swingle) stem cuttings cv. PKM1; 2022.

Rathour TP, Chandana MR, Chethan BL, Panda M, Harini K, Ranjit K, Maheshwari RV. Influence of plant bio-regulators and growing media on vegetative propagation of Citrus species: A review; 2023.

Rehman MA, Ehsan HM, Ashraf T, Gurmani ZA, Khan S, Ali M. Effect of growing media on plant growth of rough lemon (Citrus jambhiri Lush.) and Poncirus (Citrus trifoliata). Journal of Innovative Sciences. 2020;6(2):2-10.

Ruchitha T, Poojashree S. Impact of plant growth regulators in propagation of fruit crops: A review. Journal of Pharmacognosy and Phytochemistry. 2021;10(1):838-842.

Samaradiwakara SD, Champa WAH, Eeswara JP. Preharvest foliar spray of plant growth regulators expands the harvest season and improves the fruit quality of acid lime (Citrus aurantifolia (Christm) Swingle). Journal of Horticulture and Postharvest Research. 2023;6(2): 207-220.

Shanker K, Misra S, Topwal M, Singh VK. Research review on the use of different rooting media in fruit crops. Journal of Pharmacognosy and Phytochemistry. 2019;8(5):258-261.

Sharma MR. Effect of plant growth regulators and different growing media on propagation of fruit crops; 2022.

Singh S, Kukal SS, Dubey RK. Water retention and transmission characteristics of containerized growing media amended with differential proportions of compressed coir bricks. Current Science. 2019;116(9):1580-1587.

Suthar DP, Kotecha AV, Thounaojam AS. Effect of growing media and gibberellic acid on seed vigor and survival of acid lime (Citrus aurantifolia Swingle.) cv. Kagzi lime. IJCS. 2021;9(4): 291-293.

Ullah MA. Comparative Effectiveness of Different Growth Media on Growth, Biomass and Morphology Features of Kinnow Mandarin Seedling. Food Proc Nutr Sci. 2020;1(1):1-7.

Upadhayay PK, Kharal S, Shrestha B. Effect of indole-butyric acid (IBA) and wounding on rooting ability and vegetative characteristics of apple rootstock cuttings under Nepal conditions. Journal of Agricultural Science and Practice. 2020;5(4):184-192.

Vashisth T, Oswalt C, Zekri M, Alferez F, Burrow JD. 2023–2024 Florida Citrus Production Guide: Plant Growth Regulators: CPG ch. 2023;19:HS1310/CMG17, rev. 5/2023. EDIS.

Vega A, O’Brien JA, Gutiérrez RA. Nitrate and hormonal signaling crosstalk for plant growth and development. Current Opinion in Plant Biology. 2019;52:155- 163.

Verma T, Bhardwaj S, Singh J, Kapoor D, Prasad R. Triacontanol is a versatile plant growth regulator in overcoming the negative effects of salt stress. Journal of Agriculture and Food Research. 2022;100351.

Vibhute M, Tripathi MK, Tiwari R, Tiwari S, Tripathi N, Sharma M, Tomar YS, Tiwari S. Massive in vitro propagation from cultured nodal segment of three Citrus species. book: Research Aspects in Biological Science. 2022;6:102-127.

Zulia C, Gustianty LR. Response Of Natural Zpt Generation of Green Bean Sprouting Extract And Plant Media On The Growth Of Lemon Orange (Citrus Limon L.) Plant Cuttings. Journal of Scientific Research, Education, and Technology (JSRET). 2023;2(1):369-377.