Screening of Zinc Solubilizing Plant Growth Promoting Rhizobacteria (PGPR) as Potential Tool for Biofortification in Rice
Journal of Experimental Agriculture International,
Aims: Plant growth-promoting rhizobacteria enhance growth by making plant nutrients available to plants under a variety of growing conditions. The study was designed to screen zinc (Zn) solubilizing rhizobacteria and test their colonization ability in the rice rhizosphere.
Place and Duration of Study: The experiments were conducted in the Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh-2202, from January 2019 to July 2019.
Methodology: Initially, thirty-two previously isolated rhizobacteria were used for screening Zn solubilizing ability under a solid medium. Selected rhizobacteria from previous screening were used to quantify Zn solubilization in broth culture and evaluate their root colonization in rice using water agar media in a growth chamber. Early seedling growth was monitored for two weeks, and root-shoot lengths were recorded. Eleven of the tested rhizobacteria responded positively to ZnO-containing media.
Results: The Zn solubilizing index (ZSI) of the positive isolates ranged between 1.08-2.25 after 5 days of incubation. Isolate MQ1 solubilized the highest Zn both in solid medium (ZSI=2.25) and culture broth (solubilized 220.20 ppm Zn). The colonization of bacteria in the root zone was also investigated via bio-primed rice seeds of Binadhan-20. Eight of 11 isolates (MQ1, MQ2, MQ3, MQ4, OSbr5, OSbr6, EC1, and MQL9) showed substantial colonization in the rhizosphere after two weeks. The germination percentage of bio-primed seeds was not increased over the control, however, in most cases, bio-priming boasted early seedling growth. The OSbr5, OSbr6, and MQ6 isolates were superior performers in case of root and shoot growth.
Conclusion: The study identified some Zn solubilizing isolates, revealed their root colonizing ability, and observed early plant growth promotion. These isolates could be used as a potential tool for the Zn biofortification approach in rice.
- Plant growth-promoting rhizobacteria
- Zn solubilization
- root colonization
How to Cite
Black RE, Allen LH, Bhutta ZA, Caulfield LE, de Onis M, Ezzati M, et al. Maternal and child undernutrition: Global and regional exposures and health consequences. The Lancet. 2008; 371(9608):243–60.
Gibson RS. Zinc deficiency and human health: Etiology, health consequences, and future solutions. Plant Soil. 2012;361(1): 291–9.
Cakmak I, Pfeiffer WH, McClafferty B. Review: Biofortification of durum wheat with zinc and iron. Cereal Chemistry. 2010;87(1):10–20.
Welch RM, Graham RD. Breeding for micronutrients in staple food crops from a human nutrition perspective. Journal of Experimental Botany. 2004;55(396):353–64.
Gontia-Mishra I, Sapre S, Sharma A, Tiwari S. Alleviation of Mercury Toxicity in Wheat by the Interaction of Mercury-Tolerant Plant Growth-Promoting Rhizobacteria. J Plant Growth Regul. 2016;35(4):1000–12.
Iqbal U, Jamil N, Ali I, Hasnain S. Effect of zinc-phosphate-solubilizing bacterial isolates on growth of Vigna radiata. Ann Microbiol. 2010;60(2):243–8.
Cakmak I. Enrichment of cereal grains with zinc: Agronomic or genetic biofortification? Plant Soil. 2008;302(1):1–17.
White PJ, Broadley MR. Biofortification of crops with seven mineral elements often lacking in human diets – iron, zinc, copper, calcium, magnesium, selenium and iodine. New Phytologist. 2009;182(1):49–84.
Asha MN, Rahman A, Quadir QF, Islam MS. Isolation and screening of multifunctional rhizobacteria from the selected sites of Madhupur, Narshingdi and Mymensingh, Bangladesh. Research in Agriculture Livestock and Fisheries. 2015;2(1):1–8.
Choudhary DK, Sharma KP, Gaur RK. Biotechnological perspectives of microbes in agro-ecosystems. Biotechnol Lett. 2011;33(10):1905–10.
Islam MT, Deora A, Hashidoko Y, Rahman A, Ito T, Tahara S. Isolation and Identification of potential phosphate solubilizing bacteria from the rhizoplane of Oryza sativa l. cv. BR29 of Bangladesh. Zeitschrift für Naturforschung C. 2007;62(1–2):103–10.
Meena VS, Maurya BR, Bahadur I. Potassium solubilization by bacterial strain in waste mica. Bangladesh Journal of Botany. 2014;43(2):235–7.
Meena VS, Verma JP, Meena SK. Towards the current scenario of nutrient use efficiency in crop species. Journal of Cleaner Production. 2015;102:556–7.
Rahman A, Talukder NM, Islam MT. Screening phosphate solubilizing bacteria from rhizoplane of tomato. Bangladesh Journal of Progressive Science and Technology. 2005;4:1–6.
Rahman MS, Quadir QF, Rahman A, Asha MN, Chowdhury MAK. Screening and characterization of phosphorus solubilizing bacteria and their effect on rice seedlings. Research in Agriculture Livestock and Fisheries. 2014;1(1):27–35.
Taher AM, Quadir QF, Zakir HM, Nazneen T, Rahman A. Screening and isolation of arsenic tolerant Rhizobacteria from arsenic contaminated areas of Bangladesh. Progressive Agriculture. 2019;30:17– 25.
Rana A, Joshi M, Prasanna R, Shivay YS, Nain L. Biofortification of wheat through inoculation of plant growth promoting rhizobacteria and cyanobacteria. European Journal of Soil Biology. 2012;50:118–26.
Barbagelata PA, Mallarino AP. Field correlation of potassium soil test methods based on dried and field-moist soil samples for corn and soybean. Soil Science Society of America Journal. 2013;77(1):318–27.
Gontia-Mishra I, Sapre S, Tiwari S. Zinc solubilizing bacteria from the rhizosphere of rice as prospective modulator of zinc biofortification in rice. Rhizosphere. 2017;3:185–90.
Khatun MJ, Rahman A, Quadir QF, Rion MSI, Zakir HM. Isolation and characterization of plant associated rhizobacteria for plant growth promoting traits. Fundamental and Applied Agriculture. 2021;6(1):95–106.
Pikovskaya RI. Mobilization of phosphorus in soil in connection with the vital activity of some microbial species – ScienceOpen. Microbiologia. 1948;17:362–70.
Abdul-Baki AA, Anderson JD. Vigor determination in soybean seed by multiple criteria1. Crop Science. 1973;13(6): cropsci1973.0011183X001300060013x.
Alexander M. Introduction to soil microbiology. New York: Wiley; 1961.
Jones DL, Darrah PR. Role of root derived organic acids in the mobilization of nutrients from the rhizosphere. Plant Soil. 1994;166(2):247–57.
Saravanan VS, Kumar MR, Sa TM. Microbial Zinc Solubilization and Their Role on Plants. In: Maheshwari DK, editor. Bacteria in Agrobiology: Plant Nutrient Management [Internet]. Berlin, Heidelberg: Springer. 2011 [cited 2021 Sep 4];47–63.
Chang HB, Lin CW, Huang HJ. Zinc-induced cell death in rice (Oryza Sativa L.) roots. Plant Growth Regul. 2005;46(3):261–6.
Wakatsuki T. Metal oxidoreduction by microbial cells. Journal of Industrial Microbiology. 1995;14(2):169–77.
Franz A, Burgstaller W, Schinner F. Leaching with Penicillium simplicissimum: Influence of metals and buffers on proton extrusion and citric acid production. Applied and Environmental Microbiology. 1991;57(3):769–74.
Saravanan VS, Madhaiyan M, Thangaraju M. Solubilization of zinc compounds by the diazotrophic, plant growth promoting bacterium Gluconacetobacter diazotrophicus. Chemosphere. 2007;66(9): 1794–8.
Silva HSA, Romeiro R da S, Mounteer A. Development of a root colonization bioassay for rapid screening of rhizobacteria for potential biocontrol agents. Journal of Phytopathology. 2003; 151(1):42–6.
Mafia RG, Alfenas AC, Ferreira EM, Binoti DHB, Mafia GMV, Mounteer AH. Root colonization and interaction among growth promoting rhizobacteria isolates and eucalypts species. Rev Árvore. 2009;33:1–9.
Stanley NR, Lazazzera BA. Environmental signals and regulatory pathways that influence biofilm formation. Molecular Microbiology. 2004;52(4):917–24.
Lugtenberg BJJ, Kravchenko LV, Simons M. Tomato seed and root exudate sugars: composition, utilization by Pseudomonas biocontrol strains and role in rhizosphere colonization. Environmental Microbiology. 1999;1(5):439–46.
Patten CL, Glick BR. Role of Pseudomonas putida indole acetic acid in development of the host plant root system. Applied and Environmental Microbiology. 2002;68(8):3795–801.
Brown ME. Plant growth substances produced by micro-organisms of soil and rhizosphere. Journal of Applied Bacteriology. 1972;35(3):443–51.
Hussain A, Krischke M, Roitsch T, Hasnain S. Rapid determination of cytokinins and auxin in cyanobacteria. Curr Microbiol. 2010;61(5):361–9.
Rahman A, Sitepu IR, Tanf SY, Hashidoko Y. Salkowski’s reagent test as a primary screening index for functionalities of rhizobacteria isolated from wild dipterocarp saplings growing naturally on medium-strongly acidic tropical peat soil. Bioscience, Biotechnology, and Biochemistry. 2010;74(11):2202–8.
Abstract View: 115 times
PDF Download: 20 times