Open Access Original Research Article

Improvement of Genetic Purity of Breeder Seed by Introducing Line Cultivation Method for Myanmar’s Major Rice Cultivars

Tomoyuki Fujii, Yoshiyuki Yamagata, Tin Tin Myint, Yasufumi Kunihiro, Yuji Matsue, Thidar Win, Zaw Moe Aung, Win Sander Htay, Hideshi Yasui, Atsushi Yoshimura, Kazuo Ogata

Journal of Experimental Agriculture International, Page 1-13
DOI: 10.9734/jeai/2020/v42i430494

Aims: Since the genetic purity of rice seed significantly affects paddy yield and quality, many rice production countries have been attempting to improve seed quality. This study aimed to demonstrate the effectiveness of breeder seed production using line cultivation to ensure traceability of ancestral information by line and individual selection, to improve the genetic purity of breeder seed in Myanmar that has been degraded due to pedigree mixtures.

Study Design: Observational and analytic study design was used to evaluate the effect of the introduction of line cultivation method in practical activities of breeder seed production in Myanmar under the project for improvement of seed purity of breeder seed.

Place and Duration of Study: Department of Agricultural Research at Yezin, Ministry of Agriculture, Livestock and Irrigation, Myanmar, from June 2012 to December 2016.

Methodology: Using nine major rice cultivars in Myanmar, the line cultivation method was evaluated through measurement of the standard deviation and the variance component ratio in heading date, culm length, panicle length, and panicle number from 2012 to 2016. DNA polymorphism analysis by simple sequence repeat (SSR) markers was also conducted using breeder seed of the Sinthukha variety multiplied in 2013, 2014, 2015, and 2016 for the evaluation.

Results: Standard deviations of heading date, culm length, panicle length and panicle number in 2016 were significantly decreased compared to the first year of the introduction of the line cultivation method. Average heading duration among sister lines of all varieties was reduced from 8.25 days in 2013 to 5.25 days in 2016, and the uniformity of heading time among sister lines was improved. The variance component ratio of each trait in 2016 was the highest since 2013. The analysis of breeder seed by SSR markers revealed that the DNA polymorphism ratio of Sinthukha seed in 2016 was lower than that of 2013. This demonstrated that Sinthukha seed in 2016 had improved genetic purity. Consequently, it is considered that other cultivars multiplied by the line cultivation method have improved their genetic purity as well.

Conclusion: Line cultivation is an effective method to improve genetic purity and maintain genetic stability of the breeder seed in Myanmar’s rice cultivars. Genetically pure breeder seed would improve quality of downstream seed such as certified seed. Consequently, it is expected that productivity and quality of rice will be improved thus income of farmers will increase.

Open Access Original Research Article

In vitro Regeneration and Callogenesis of Libidibia ferrea

Daniel da Silva, Angela Maria Imakawa, Kamylla Rosas Vieira Guedes, Flávio Mauro Souza Bruno, Paulo de Tarso Barbosa Sampaio

Journal of Experimental Agriculture International, Page 14-24
DOI: 10.9734/jeai/2020/v42i430495

Libidibia ferrea (Fabaceae) is a valuable medicinal species in the Amazon, but as it is a protected plant, collection from natural populations is forbidden. Therefore, establishing an efficient system for in vitro regeneration and to improve callogenesis of this species is desirable. To determine the optimal nutritional factors needed for shoot multiplication and callus induction, different culture media, plant growth regulators and LED light sources were tested. The data were subjected to analysis of variance (ANOVA) and means compared by Tukey’s test at p < 0.05. We observe that explants inoculated in the Murashige and Skoog (MS) media with 0.05 mg L-1 of 6-benzilaminopurine (BAP) and cultivated under red-blue LED induced the highest number of shoots (3.67), number of buds (3.13), multiplication rate (15.67) and shoots length (22.03 mm) when compared with other treatments. MS and B5 media supplemented with 2.21 and 4.42 mg L-1 of 2,4-D induced 100% formation of friable callus cultivated under red-blue LED, demonstrating that the light quality significantly influenced callogenesis. Obtained results confirmed that in vitro regeneration and callogenesis is a useful strategy in the protection of endangered species. In this way, a new renewable source of biomass with high quality plant material is presented aiming at the bioprospecting of seedling extracts and friable callus to obtain secondary metabolites of this medicinal plant.

Open Access Original Research Article

The Effect of Plant Densities and Different Maturity Types on Maize Grain and Fodder Yield

Boakye Boateng Augustine, Atta Boateng Bright

Journal of Experimental Agriculture International, Page 25-32
DOI: 10.9734/jeai/2020/v42i430496

Three maturity types of maize, Abontem (extra early maturing), Omankwa (early maturing) and Obaatanpa (medium maturing) were evaluated at three different plant densities 66,667 plants ha-1, 100,001 plants ha-1 and 133,334 plants ha-1  to determine the optimum plant density for grain and fodder yield. The experiment was laid   in randomized complete block with four replicates factorial fashion. The maize maturity type had significant influence on all the traits measured. Plant density had significant effect on cob width, grain and fodder yield but no significant influence on plant height, days to 50% anthesis and silking, anthesis and silking interval, cob length and 1000 grain weight. Obaatanpa (medium maturing) was the maize maturity type that produced the highest grain yield (3139 kg ha-1) and fodder yield of (4173 kg ha-1).  Obaatanpa with 133,334 plants ha-1 interaction produced the highest grain and fodder yield with 3186 kg ha-1 and 4240 kg ha-1 respectively. Obaatanpa with 133,334 plants ha-1 would be recommended for commercial grain and fodder production because the high yielding potential as observed in the study.

Open Access Original Research Article

Productive Increase in Soybeans from Hormone Treatment, Inoculation and Co-inoculation

Luiz Leonardo Ferreira, Neusmar Rodrigues Valentim Júnior, João Carvalho de Morais Neto, Ivan Ricardo Carvalho, Marilaine de Sá Fernandes, Ariana Bertola Carnevale, Núbia Sousa Carrijo dos Santos, Carmen Rosa da Silva Curvêlo, Alexandre Igor de Azevedo Pereira

Journal of Experimental Agriculture International, Page 33-43
DOI: 10.9734/jeai/2020/v42i430497

The increase in productivity in soybean cultivation has been achieved by many Brazilian producers, due to the technological advances related to soil management, mineral nutrition, pest and disease control, genetic improvement, and the use of beneficial microorganisms via inoculation and co-inoculation. However, the application of inoculants based on Bradyrhizobium japonicum and Azospirillum brasilense still needs to be better studied among the many soybean cultivars available in the market. Therefore, the objective of this study is to evaluate the production increase in three soybean cultivars, through the application of synthetic hormones, inoculant and co-inoculant. The experimental design was a randomized complete block design with four repetitions, in a 3x6 factorial scheme, with three soybean cultivars (Desafio 8473, TMG 1180 and CD 2737) and six seed treatments (Control, Inoculation, Hormonal, Co-inoculation, Co-inoculation + Hormonal and Inoculation + Hormonal). The treatments with the diazotrophic bacteria (Inoculation + Co-inoculation) and Hormonal did not influence the yields of cultivars 'CD 2737' and 'Desafio 8473', which can only be cultivated with the standard seed treatment of the region. The 'TMG 1180' cultivar has it’s yield increased when it’s seeds are inoculated with Hormonal seed treatments and with diazotrophic bacteria in Co-inoculation.

Open Access Original Research Article

Characterization of Biochars Derived from Different Organic Wastes

Md. Shahin Hossin, Morsheda Akter Mukta, Md. Abdur Rouf Talukder, Md. Mustafizur Rahman, Md. Sagirul Islam Majumder, Md. Rafiq Uddin

Journal of Experimental Agriculture International, Page 44-50
DOI: 10.9734/jeai/2020/v42i430498

Biochar is widely recognized as an efficient tool for soil fertility and carbon sequestration. The understanding of its physical and chemical properties is strongly related to the type of used material, is crucial to identify the most suitable application of biochar in soil. A selection of organic wastes (e.g., rice straw, rice husk and saw dust) with different characteristics were pyrolyzed at 350 0C in order to optimize the physical and chemical properties of biochar as a soil amendment. Bulk density of biochar samples in this study varied from 0.18 mg/m3 to 0.22 mg/m3. Moisture content was in the order of saw dust (8.41%) > rice straw (7.21%) > rice husk (6.74%). Rice straw biochar had highest pH (8.80) and electrical conductivity, EC (3.45 ds/m). The organic carbon was highest in rice straw biochar (46.50%) followed by with rice husk (39.3%) and saw dust (25.3%). The total nitrogen contents of the rice straw, rice husk and saw dust were 1.78%, 1.59% and 1.11%, respectively. The rice straw biochar contents higher available P, as compared to rice husk and saw dust biochar. The rice straw biochar had higher exchangeable Ca (5.60 meq/100 g), Mg (1.70 meq/100 g). This study suggested that the rice straw biochar has a great potential as soil amendment among the three biochars for sustainable soil management.

Open Access Original Research Article

Influence of Phosphorus on Initial Growth and Production of Fresh Biomass of Legumes

Sebastiana Renata Vilela Azevedo, Geovana Gomes de Sousa, Maria Beatriz Ferreira, Marcelo Pereira Dutra Júnior, Iara Cristina Araujo Rocha, Jaqueline Rocha de Medeiros, Marília Gabriela Caldas Pinto, Rivaldo Vital dos Santos

Journal of Experimental Agriculture International, Page 51-59
DOI: 10.9734/jeai/2020/v42i430499

Aims: To evaluate whether the initial growth and production of green biomass of the legume species Crotalaria juncea L. and Canavalia ensiformis (L.) DC. are influenced by phosphate fertilizer.

Study Design: A completely randomized design was used in a scheme 05 treatments x 02 cultures, with 3 replications, totaling 30 vessels in the experiment.

Place and Duration of Study: Forest nursery the Federal University of Campina Grande, campus of Patos-PB, between April 2018 and May 2018.

Methodology: The treatments consisted of two legumes (C. juncea and C. ensiformis) and five doses of phosphorus (00-50-100-150-200 mg kg-1 of P2O5) through single super phosphate. Fortnightly measurements of height and diameter were performed for 60 days. Fresh biomass of the shot and root was obtained at the end of the evaluations.

Results: There is a higher growth in height for individuals of C. ensiformis when cultivated in doses of 150 mg kg-1, with an average of 30.68 cm. For C. juncea, the treatments were similar. For the stem diameter, there was significant interaction (p <0.05) only for C. juncea, in which, unlike the results obtained for height, this variable grew linearly with increased phosphorus doses. Regardeless of the P doses fresh biomass production of C. ensiformis was higher than to C. juncea. Already in function on the levels of P there is an influence of nutrient only for C. ensiformis, with higher total biomass production and when cultivated with 100 mg kg-1 of P2O5.

Conclusion: Regardless of the cultivated species phosphorus influences the initial growth and production of fresh biomass. In general, it is recommended to cultivate the legumes studied with doses of 100 mg kg-1 of P2O5 through single super phosphate for a higher production of total fresh biomass.

Open Access Original Research Article

Components of the Production and Chemical Composition of Safflower Seeds as a Function of Nitrogen Fertilization

Fernando Luiz da Cruz Balena, Reginaldo Ferreira Santos, Doglas Bassegio, Luciene Kazue Tokura, Jerry Adriani Johann, Luiz Antônio Zanão Júnior

Journal of Experimental Agriculture International, Page 60-70
DOI: 10.9734/jeai/2020/v42i430500

Aims: The objective of this study was to evaluate the effects of nitrogen fertilizer application on the direct and indirect productive components of safflower.

Study Design: The treatments were arranged in randomized blocks, with four replicates, and consisted of five doses of nitrogen (N): 0, 50, 100, 150 and 200 kg.ha-1, in the form of urea (45% N), which was manually incorporated into the soil 30 days after plant emergence.

Place and Duration of Study: Sowing was carried out under field conditions on a small rural property in the municipality of Toledo-PR, Brazil. The experiment was conducted in between May and October 2018, totaling 158 days.

Methodology: Morphometric parameters were evaluated at the full flowering stage, and the yield and chemical composition of the achenes were assessed during physiological maturation of the plants. Data were analyzed using analysis of variance (ANOVA) at the 5% significance level. Regression equations were fitted to the means of the quantitative variables.

Results: The results showed that safflower grain yield was strongly influenced by the application of N-urea, with the maximum yield at a dose of 120 kg.ha-1 N. A joint analysis of the regression equation for N-urea application and grain yield, and the prices of N and safflower grains used, revealed that the most economical dose was 70 kg.ha-1 N, which provided the maximum profit per unit area. There was a positive linear correlation between grain yield and protein content (r = 0.51). Stem diameter, aerial dry biomass, grain yield, number of branches, and floral chapters per plant increased with N application, indicating that safflower culture responds positively to nitrogen fertilization.

Conclusion: We conclude that sustainable use of nitrogen fertilizer using the maximum economic efficiency dose, can contribute to better plant health and, at the same time, reduce production costs.

Open Access Original Research Article

Screening of Rice Lines (Oryza spp L. 1753) for Salinity Tolerance at Vegetative Stage under Senegal River Valley Conditions

Yacine Maïga, Gbenonchi Mawussi, Omar Ndaw Faye, Abdoulaye Fall

Journal of Experimental Agriculture International, Page 71-81
DOI: 10.9734/jeai/2020/v42i430501

Rice (Oryza spp. L., 1753) is the most staple food cereal in Senegal. However, national production is low and cannot cover the country needs. Salinity limits rice production in Senegal River valley which is main rice growing area in Senegal. This study assessed salinity tolerance of 30 rice lines at vegetative stage in station of Senegalese Institute for Agricultural Research (ISRA) at Ndiol in Senegal River valley. Experimental design adopted was alpha lattice with three rehearsals. Observations were performed on plant height, main stem development and number of tillers. The level of salinity and pH in surface water, groundwater and soil were monitored. The results indicate that salinity affects plant height and tillering ability and among 30 lines, four were identified highly tolerant to salinity at vegetative stage. These are the varieties HK11-NDIOL-11-LON-1, D20-ART20-ARS-1, D56-ARS-NCRIB-1-1 and HK72-NIONO-5-1-1. The decision to incorporate these varieties in salinity tolerance breeding programs would depend on salt tolerance screening across all growing stages.

Open Access Original Research Article

Susceptibility of Bagged Guavas to the Attack of Fruit Flies (Tephritidae)

Adalton Raga, Ester Marques de Sousa, Sara Braga e Silva, Léo Rodrigo Ferreira Louzeiro

Journal of Experimental Agriculture International, Page 82-87
DOI: 10.9734/jeai/2020/v42i430502

Fruit bagging is an important strategy to protect fruit against fruit fly (Tephritidae) infestation and pesticide contamination. In laboratory, we compared the forced infestation of guavas by Ceratitis capitata (Wiedemann) and Anastrepha fraterculus (Wiedemann) when wrapping them with non- woven fabric (NWF) bags. The combined repellence and protection effects of white, green, blue and red NWF bags were tested in comparison with un-bagged guavas. Bagged fruit with the NWF tissue stuck to the pericarp were separately exposed to both fruit fly species for 48 hours, with a ratio of 10 females per fruit inside of laboratory cages. In C. capitata, the number of pupae per fruit was significantly higher in the green NWF bags, and the infestation in the other colour bags were similar to the control group (non-bagged). In A. fraterculus, guavas with red NWF bags had significantly higher infestation (205.42 pupae per fruit) than fruit that received the other colour bags White and blue bags were less attractive for oviposition from both fruit fly species. The adherence of the NWF bags to the fruit surface causes egg-laying of fruit flies.

Open Access Original Research Article

Influence of Time of Cowpea Introduction at the Establishment Phase of Pineapple on Performance of Pepper in a Pineapple-Pepper-Cowpea Intercropping System

Ademola Johnson Ajayi, Samuel Ohikhena Agele, O Peter Aiyelari

Journal of Experimental Agriculture International, Page 88-98
DOI: 10.9734/jeai/2020/v42i430503

Cowpea was sown into pineapple-pepper intercrop at the establishment of pineapple in 2011 and 2012 to evaluate the effects of its time of sowing into pineapple-pepper intercrop on growth and yield components of pepper. In 2011, cowpeas were sown into pineapple-pepper intercropped plots at 3 week intervals in addition to the sole crop components of pepper, cowpea and pineapple. In 2012, the experiment was repeated with the inclusion of pineapple-pepper intercrop with no cowpea introduced. The experiments were laid out in a randomized complete block design with three replications.

Early sowing of cowpea at the time of transplanting pepper and at 3 weeks after transplanting (WAP) pepper significantly reduced pepper leaf area by 97.5 and 80.1% respectively. The decline in the number of fruits per plant ranges from 90.4% with sowing of cowpea at transplanting of pepper to 35.6% sowing was delayed by 9 weeks in 2011 while decline in 2012 rages from 89.6 to 1.4%. Lower fruit weight decline was obtained with delayed introduction of cowpea with 29.1 and 14.5% decline when cowpea was sown 6 and 9 WAT respectively. It is recommended that pepper could be introduced into pineapple field at establishment phase. However, sowing of cowpea should be delayed up to six weeks after transplanting pepper in order to reduce competition and to enhance complementary use of resources by the component crops.

Open Access Original Research Article

Economics of Yam Production in Gboyin Local Government Area of Ekiti State, Nigeria

O. C. Ariyo, M. B. Usman, M. M. Olorukooba, O. E. Olagunju, O. B. Oni, R. Suleiman, A. J. Adetunji, M. O. Ariyo

Journal of Experimental Agriculture International, Page 99-110
DOI: 10.9734/jeai/2020/v42i430504

The study of economics of yam production was carried out in Gboyin Local Government Area of Ekiti State, Nigeria. The specific objectives are to: describe the socio-economic characteristics of yam farmers, determine and analyse the profitability of yam production, determine the input and output relationship of yam production and identify constraints to yam production. Three- stage sampling procedure was used to obtain information from respondents. A total number of 140 respondents selected randomly from four towns (Ode- Ekiti, Agbado, Aisegba and Ilumoba) were used for the study. Data were collected with interview schedule administered to obtain information from the farmers. Descriptive statistics, budgetary, profitability and multiple regression analysis were used to analyse the data. The result of the study showed that both males and females are involved in yam production in the study area with mean age of 45 years and mean household size of 5 members. The mean faming experience and farm size was 10 years and 1.83 hectares respectively. Majority (83.57%) had formal education (primary, secondary and tertiary education) and had no access to loans. The result of budgetary analyses showed that yam production is profitable with an average net farm income of N 91, 876.50 per production season. The profitability indicators revealed that the enterprise is viable and worthwhile. The coefficient of both fertilizer and farm size were significant at (P< 0.001), labour at (P< 0.05) while the coefficient of seed was significant at (P< 0.10) probability level. These variables are very vital and crucial in yam production. Inadequate capital and planting materials, high cost and inaccessibility to inputs, and poor produce price etc are the problems of yam production in the study area. It was concluded that yam production is a profitable, viable and worthwhile enterprise which can be embarked upon by both the youths and adults of the study area.

Open Access Original Research Article

Induction of Variability in Three Nigerian Pepper Varieties using Gamma Irradiation

N. E. Abu, E. O. Ojua, O. U. Udensi

Journal of Experimental Agriculture International, Page 111-119
DOI: 10.9734/jeai/2020/v42i430505

This study was aimed at inducing mutation on three cultivars of Capsicum annuum L. to source for new and favourable genetic variations that could be exploited for better agronomic traits. The mutation was induced by exposing the seeds of Shombo, Tatase and Nsukka yellow pepper to varied dosages (0, 50, 100, 150 and 200 Gy from 60Co source) of gamma irradiation. The study was conducted in the Botanic Garden of the University of Nigeria. Seeds exposed to 50Gy produced significantly (P < 0.05) the highest number of fruits per plant, highest fruit yield and improved morphological traits in Shombo. Higher dosages of irradiation gave lower fruits per plant and yields than untreated seeds in Shombo. Tatase irradiated with 150 Gy had significantly the highest single fresh fruit weight. Generally in Tatase 50, 100 and 150Gy increased some morphological traits significantly (P < 0.05). Although 50 Gy gave highest number of fruits per plant, 100, 150 and 200Gy had significantly higher fruit yield with yield increasing as dosage increased. For Nsukka yellow, irradiation did not show a consistent pattern in improving morphological traits. But 200Gy produced significantly the highest number of fruits per plant and highest yield. Gamma irradiation dosage of 50 – 200 Gy can be exploited to create variability for improvement of morphological and yield traits in pepper plants.

Open Access Original Research Article

Plug Cell Volume, Main Temperature and the Synthetic Cytokinin 6, Benzyl Aminopurine (BAP) Spray on Lettuce Biomass Accumulation during Nursery at Ambient Temperatures

J. Chertok, D. Carnelos, J. Lozano Miglioli, P. Fujinuma, E. Giardina, A. Di Benedetto

Journal of Experimental Agriculture International, Page 120-135
DOI: 10.9734/jeai/2020/v42i430506

Although nursery lettuce information is plentiful, the use of exogenous cytokinin (6, benzyl amino purine, BAP) as a plant growth regulator, which would increase vegetable nursery yield has only recent suggested. On the other hand, biomass relationships between plug cell volumes, BAP uses and main temperature during nursery is quite scarce. Our results showed that the growth response of lettuce seedlings during nursery is firstly related to mean air temperature, independently the environmental characteristics of the cultivar tested. From the view of a nursery grower, yield can be optimize when higher plug cell number per tray was used or a BAP spray was applied to limited plug trays (128- or 288-cells). One of the novelty result of this work reside in the objective protocol proposed (the marginal root dry weight accumulation rate) to decide the time to transplant. Finally, our results showed that plug purchaser would be change their visual criteria when they buy BAP-treated lettuce for increased field yield.

Open Access Original Research Article

Diversity Studies in Vigna Species and Their Implications in Further Breeding

Abolade Oluremi Bolaji, Atanda Samuel Oladejo, Samuel Ebeagu

Journal of Experimental Agriculture International, Page 136-144
DOI: 10.9734/jeai/2020/v42i430508

Aims: To evaluate, identify and select superior genotypes with better agronomic traits with respect to insect resistance and yield components for the development of improved cowpea varieties.

Study Design: The plants were raised using completely randomized design (CRD).

Place and Duration of Study: Screen house of the Department of Botany, Obafemi Awolowo University, Ile-Ife, Nigeria, between between July 2017 and January 2018.

Methodology: A total of 67 Vigna accessions consisting of 36 V. vexillata and 31 V. unguiculata species were evaluated and the quantitative and qualitative attributes of the various accessions studied were documented. The data obtained in the study were subjected to univariate analysis involving descriptive statistics, as well as multivariate analysis involving the General Linear Model (GLM) Analysis of Variance (ANOVA) and Principal Component Analysis (PCA). The differences among means of each variable were tested using the Duncan Multiple Range Test (DMRT) at ˂0.05 using System Analysis Software (SAS) version 9.13. Pearson Correlation Coefficient was also used to test the correlation among the quantitative attributes of the varieties studied.

Results: The results of the investigation showed that there was significant variation in most of the quantitative attributes of the species studied though many of the qualitative vegetative attributes were considerably uniform. The Principal Component Analysis showed that some of the important traits that contributed significantly to the variation were number of days to seedling emergence, plant height, leaf length, leaf breadth, petiole length, pod length, pod breadth, seed length, seed breadth, number of seeds per pod and number of locules.

Conclusion: From the findings obtained in this study, it could be concluded that the desirable traits of Tvnu1249 (V. vexillata) such as early maturity, and Tvnu72 (V. vexillata) with high percentage seed set and resistance to flower bud thrips could be transferred into Sanzi and Ifebrown (V. unguiculata varieties) with larger seed sizes and high percentage seed set through hybridization and other breeding techniques.

Open Access Original Research Article

Amegilla sp. (Hymenoptera: Apidae) Visitors to Cotton (Gossypium hirsutum, Malvaceae) Flowers and Their Role in Crop Pollination at Djoumassi (North, Cameroon)

Adamou Moïse, Mazi Sanda, . Taimanga, Yatahaï Clément Minéo

Journal of Experimental Agriculture International, Page 145-157
DOI: 10.9734/jeai/2020/v42i430509

Aims: To evaluate the impact of a single flower visit of Amegilla sp. on the production of Gossypium hirsutum.

Study Design: A randomized complete block design was used in a split plot scheme of 437 m2 delimited and divided into eight subplots, each measuring 8*4.5 m2.

Place and Duration of Study: Locality of Djoumassi, Benoue Division, in the city of Garoua, the North Region of Cameroon from June through October 2018 and 2019.

Methodology: The experiments were carried out on 540 flowers labeled at bud stage and divided in four treatments: two differentiated according to the presence or absence flowers protection regarding insect visits; the third protected and uncovered when flowers were opened, to allow Amegilla sp. visits and the fourth with the flowers protected, uncovered when they were opened, then rebagged without any visit.

Results: Among 21 insect species recorded on G. hirsutum flowers, Amegilla sp. ranked second and harvested nectar and pollen. Throughout the pollination efficiency of a single flower visit, Amegilla sp. provoked a significant increase of the fruiting rate, the mean number of seeds per boll and the percentage of normal seeds by 14.89%, 12.93% and 7.01% respectively.

Conclusion: The conservation and installation of Amegilla sp. nests close to G. hirsutum fields is recommended to improve its boll production and seed quality.