Phosphite-based Products in the In vitro Colletotrichum musae Control
Maria Luísa Mendes Rodrigues *
State University of Montes Claros, Janaúba, Brazil.
Edson Hiydu Mizobutsi
State University of Montes Claros, Janaúba, Brazil.
Paola Junayra Lima Prates
State University of Montes Claros, Janaúba, Brazil.
Paula Virgínia Leite Duarte
State University of Montes Claros, Janaúba, Brazil.
Regina Cássia Ferreira Ribeiro
State University of Montes Claros, Janaúba, Brazil.
Juceliandy Mendes da Silva Pinheiro
State University of Montes Claros, Janaúba, Brazil.
Gisele Polete Mizobutsi
State University of Montes Claros, Janaúba, Brazil.
Martielle Batista Fernandes
State University of Montes Claros, Janaúba, Brazil.
Luana Sabrine Silva
State University of Montes Claros, Janaúba, Brazil.
*Author to whom correspondence should be addressed.
Abstract
Aims: The aim of this study was to evaluate the in vitro effect of different phosphite formulations and concentrations on the development of Colletotrichum musae. Sample: to evaluate the inhibition of germination, mycelial growth and sporulation of Colletotrichum musae.
Study Design: Treatments were conducted in a completely randomized design, with 4 replicates, each replicate consisting of 1 Petri dish.
Place and Duration of Study: Laboratory of Post-Harvest Pathology, State University of Montes Claros, between March and October 2017.
Methodology: Three different phosphite formulations were used: FCu1 (4% Cu + 20% P2O5), FCu2 (4% Cu + 22% P2O5) at concentrations of 0.5;1.0; 1.5 and 2.0 mL L-1 and FK (42% P2O5 + 27.7% K2O) at concentrations of 0.5; 1.0; 1.5 and 2.0 mg.L-1. Products were incorporated into the respective culture media. Culture medium alone and culture medium + imazalil were used as controls. Petri dishes were housed in BOD chamber at 25°C under a 12 hours photoperiod.
Results: Results were submitted to analysis of variance and regression, and means were compared by the Tukey test (P <0.05). Control was compared to the other treatments by the Dunnet's test (P <0.05). Among the tested phosphite formulations, copper and potassium phosphites were found to reduce the mycelial growth of Colletotrichum musae. FCu2 presents a fungicide-like effect from the concentration of 0.5 m.L-1 in the control of conidia production. As for the FCu1, a fungicide-like effect was observed in the control of germination from the concentration of 1.5 mL.L-1.
Conclusion: A significant fungistatic effect was observed between the concentrations of the products in the mycelial growth, sporulation and germination obtaining control of up to 100% of the development of C. musae. Copper phosphites were as effective as fungicide in inhibiting fungal development.
Keywords: Disease, anthracnose, alternative treatment.