Micronutrient Delivery System for Induction of Organogenesis in Banana in vitro Cultures
Luciana Cardoso Nogueira Londe *
Empresa de Pesquisa Agropecuária de Minas Gerais, Rodovia MGT 122, km 155, Campo Experimental do Gorutuba, Nova Porteirinha, MG. 39.525-000, Brazil.
Wagner A. Vendrame
Department of Ornamental Horticulture, Tropical Research and Education Center, University of Florida, 18905 SW 280 th St, Homestead, FL. 33031, United States of America.
Alexandre Bosco de Oliveira
Department of Crop Science, Center of Agricultural Sciences, Universidade Federal do Ceará, Av. Mister Hull, 2977, zip code 60356-001, Fortaleza, CE, Brazil.
Maurício Mendes Cardoso
Empresa de Pesquisa Agropecuária de Minas Gerais, Rodovia MGT 122, km 155, Campo Experimental do Gorutuba, Nova Porteirinha, MG. 39.525-000, Brazil.
*Author to whom correspondence should be addressed.
Abstract
Micropropagation techniques represent one of the technologies, which allows the large-scale production of banana and the culture medium composition is one of the major factors affecting in vitro propagation of plants. The objective of this study was to evaluate the efficiency of a new micronutrient delivery system based on ionic Cu and Zn for inducing in vitro organogenesis in two banana cultivars in vitro cultures, Grand Naine and Pysang Ceylon. The first experiment evaluated different concentrations of BAM-FX® (0.16, 0.32, 0.64, 1.28, and 2.56 µl ml-1) added to the MS culture medium. The concentration of 0.16 µl ml-1 BAM-FX® provided the best results for in vitro shoot and root growth and development. Therefore, a second experiment was performed to evaluate the potential of combining BAM-FX® with a reduced concentration of MS medium (¼, ½, and ¾ strength), or the use of BAM-FX® alone without MS medium. Results indicate that MS at ¾ strength combined with 0.16 µl ml-1 BAM-FX® provided proper in vitro shoot and root growth and development. The use of BAM-FX® in vitro requires additional studies to verify the feasibility of this product for efficient micropropagation of banana, as well as for other species.
Keywords: Musa species, nutrient uptake, ionic cooper and zinc.