Multiple Sequence Alignment Reveals Diversity among Eight African Bush Mango (Irvingia gabonensis Aubry-Lecomte ex O’Rorke) Cultivars
U. G. Adebo *
Fruits and Biotechnology Research Programme, National Horticultural Research Institute, Ibadan, Nigeria and Tasmania Institute of Agriculture, University of Tasmania, Tasmania, Australia.
J. O. Matthew
Fruits and Biotechnology Research Programme, National Horticultural Research Institute, Ibadan, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Multiple sequence analysis is one of the most widely used model in estimating similarity among genotypes. In a bid to access useful information for the utilization of bush mango genetic resources, nucleotide sequences of eight bush mango (Irvingia gabonensis) cultivars were sourced for and retrieved form NCBI data base, and evaluated for diversity and similarity using computational biology approach.
The highest alignment score (26.18), depicting the highest similarity, was between two pairs of sequence combinations; BM07:BM58 and BM12:BM69 respectively, while the least score (19.43) was between BM01: BM13. The phylogenetic tree broadly divided the cultivars into four distinct groups; BM07, BM58 (cluster one), BM01 (cluster 2), BM15, BM13 and BM35 (cluster 3), and BM12, BM69 (cluster 4), while the sequences obtained from the analysis revealed only few fully conserved regions, with the single nucleotides A, and T, which were consistent throughout the evolution.
Results obtained from this study indicate that the bush mango cultivars are divergent and can be useful genetic resources for bush mango improvement through breeding.
Keywords: Breeding, genetic diversity, phylogenetic tree, cultivars, genetic variation