Effect of Different Physiological Parameters on Sporulation and Mycelial Growth of Macrophomina phaseolina Isolated from Fenugreek In Vitro
Santosh Kumari
Department of Plant Pathology, College of Agriculture, SKRAU, Bikaner -334006, Rajasthan, India.
A. K. Meena
Department of Plant Pathology, College of Agriculture, SKRAU, Bikaner -334006, Rajasthan, India.
Jasveer Singh *
Department of Plant Pathology, College of Agriculture, SKRAU, Bikaner -334006, Rajasthan, India.
Arti Jatav
Department of Plant Pathology, College of Agriculture, SKRAU, Bikaner -334006, Rajasthan, India.
*Author to whom correspondence should be addressed.
Abstract
Fenugreek (Trigonella foenum-graecum L.) also known as Methi belongs to family leguminoceae. The study of physiological parameters for the mycelial growth and sporulation of the Macrophomina phaseolina was undertaken by culturing the pathogen in different physiological parameters. Among the temperature, highest mycelium growth and sporulation was recorded at 35°C. In all the tested different lights maximum mycelial growth and sporulation of M. phaseolina was observed in black light. Among the carbon and nitrogen sources maximum mycelial growth and sporulation were observed in sucrose and L-alanine. Respectively, in all the tested different pH levels maximum mycelial growth and sporulation was recorded at 7.0 pH.
Keywords: Physiological parameters, mycelial growth, Macrophomina phaseolina, fenugreek