Isolation of Cercospora canescens and Management of Cercospora Leaf Spot (Cercospora canescens) of Mothbean through Botanicals
Rakesh Kumar
Department of Plant Pathology, College of Agriculture, Swami Keshwanand Rajasthan Agricultural University, Bikaner–334006, Rajasthan, India.
A. K. Meena
Department of Plant Pathology, College of Agriculture, Swami Keshwanand Rajasthan Agricultural University, Bikaner–334006, Rajasthan, India.
Vikash Kumar *
Department of Plant Pathology, College of Agriculture, Swami Keshwanand Rajasthan Agricultural University, Bikaner–334006, Rajasthan, India.
Jasveer Singh
Department of Plant Pathology, College of Agriculture, Swami Keshwanand Rajasthan Agricultural University, Bikaner–334006, Rajasthan, India.
*Author to whom correspondence should be addressed.
Abstract
The pathogen was isolated from diseased leaves of mothbean on potato dextrose agar by standard tissue isolation method and pathogenicity was proved following Koch's postulates. On the basis of morphological studies like mycelium was whitish to brownish, hyaline and acircular, straight to variously curved, multiseptate (2-16) many celled, thread like conidia and cultural studies the pathogen was identified as Cercospora canescens Ellis and Martin. Among eight botanicals tested at three concentrations (5, 10 and 15%) under in vitro condition against C. canescens, neem seed kernel extract was found most effective and inhibiting mycelial growth (58.00%) at 15% concentration followed by tulsi leaf extract (52.96%) and neem leaf extract (44.68%) also found effective. Least mycelial growth inhibition of C. canescens was obtained in turmeric extract (27.31%) and giloy (25.31%) extract at 15% concentration.
Keywords: Isolation, Cercospora canescens, botanicals, mothbean, Cercospora leaf spot