Studies on Association of Seed Borne Mycoflora in Different Varieties of Black Gram [Vigna mungo (L.) Hepper] by Incubation Methods
Kiran Netam *
Department of Plant Pathology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, (C.G.), 492012, India.
Nisha Toppo
Department of Plant Pathology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, (C.G.), 492012, India.
Suresh Kumar Sahu
Department of Plant Pathology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, (C.G.), 492012, India.
Gote Sachin Shankar
Department of Plant Pathology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, (C.G.), 492012, India.
Sushma Verma
Department of Plant Pathology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, (C.G.), 492012, India.
N. Khare
Department of Plant Pathology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, (C.G.), 492012, India.
*Author to whom correspondence should be addressed.
Abstract
The present study was conducted to detect and identify seed-borne pathogenic fungi in some different varieties of black gram and their effect on seed germination. Six varieties of black gram (Indira urd-1, Pratap-1, KU-96, TPU-4, TIU-22) and local variety collected from village for experiment. The Eleven seed associated mycoflora recorded were Aspergillus flavus Aspergillus niger, Fusarium spp., Alternaria spp., Rhizopus spp., Penicillium spp., Curvularia spp., Cladosporium spp., Colletotrichum spp., Chaetomium spp. and Trichoderma spp. were detected and identified from the seeds of different varieties of black gram by using the standard blotter method, agar plate method, 2,4-D blotter method, roll paper towel method and deep freeze method. In all these methods, poor germination percentage was recorded in local variety, may be due to the presence of seed associated mycoflora with maximum frequency, whereas KU-96 variety has most extreme germination percentage due to the minimum frequency of seed associated mycoflora as compared to other varieties of black gram taken in the examination.
Keywords: Black gram, seed borne mycoflora, standard blotter method, agar plate method seed germination.