The Relationship Between Body Weight and Growth Hormone Gene Polymorphism in Indigenous Turkeys of Nigeria

Mercy O. Oso *

Department of Animal Science, Animal Breeding and Genetics Unit, University of Ibadan, Nigeria and School of Biomedical Sciences, Kent State University, Ohio, USA.

Mabel O. Akinyemi

Department of Animal Science, Animal Breeding and Genetics Unit, University of Ibadan, Nigeria and Biological Sciences Department, Fairleigh Dickson University, Florham Campus, NJ 07940, USA.

Osamede H. Osaiyuwu

Department of Animal Science, Animal Breeding and Genetics Unit, University of Ibadan, Nigeria.

Olarenwaju B. Morenikeji

Division of Biological and Health Sciences, University of Pittsburgh, Bradford, USA.

Oluwatobi E. Fijabi

Department of Animal Science, Animal Breeding and Genetics Unit, University of Ibadan, Nigeria.

Michael O. Adeosun

Department of Animal Science, Animal Breeding and Genetics Unit, University of Ibadan, Nigeria.

Ibilola A. Shobanke

Department of Animal Science, Animal Breeding and Genetics Unit, University of Ibadan, Nigeria.

Kayode M. Ewuola

Department of Animal Science, Animal Breeding and Genetics Unit, University of Ibadan, Nigeria.

Temidayo E. Koledafe

Department of Microbiology, Environmental Microbiology Unit, University of Ibadan, Nigeria.

Oluwaseyi E. Oso

Department of Animal Science, Animal Breeding and Genetics Unit, Babcock University, Nigeria.

Geofrey I. Nwokorie

Department of Animal Science, Animal Breeding and Genetics Unit, University of Ibadan, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

The candidate gene approach is a valuable tool for improving breeding programs, particularly in poultry, by identifying traits for enhanced production performance. This study investigates the growth hormone (GH) gene as a potential determinant of body weight in Nigerian indigenous turkeys. DNA was extracted from 60 twelve-week-old turkeys using blood samples, and a 466bp DNA fragment was amplified via PCR with primers designed from chicken sequences. The PCR product underwent Msp1 restriction enzyme digestion and was visualized on a 1.5% agarose gel. Genotypes associated with body weight were assessed using a General Linear Model. The GH/Msp1 locus revealed Two alleles (A and G) and three genotypes (AA, AG, and GG), with allele G being predominant. Genotype AG had the highest frequency (0.55), followed by AA (0.42), and GG had the lowest frequency (0.03). However, the least square means analysis showed that the polymorphism in the GH gene of the studied population was not significantly associated with body weight (p> 0.05). Nonetheless, the polymorphic nature of the GH gene in Nigerian turkeys suggests potential for further exploration in quantitative traits.

Keywords: Polymorphism, PCR-RFLP, Growth Hormone gene (GH), Nigerian indigenous turkey, body weight


How to Cite

Oso, Mercy O., Mabel O. Akinyemi, Osamede H. Osaiyuwu, Olarenwaju B. Morenikeji, Oluwatobi E. Fijabi, Michael O. Adeosun, Ibilola A. Shobanke, Kayode M. Ewuola, Temidayo E. Koledafe, Oluwaseyi E. Oso, and Geofrey I. Nwokorie. 2024. “The Relationship Between Body Weight and Growth Hormone Gene Polymorphism in Indigenous Turkeys of Nigeria”. Journal of Experimental Agriculture International 46 (7):75-80. https://doi.org/10.9734/jeai/2024/v46i72560.

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