Mutation Breeding and Its Importance in Modern Plant Breeding: A Review

Shahzeb Ali *

Department of Genetics and Plant Breeding, Lovely Professional University, Punjab, India.

Talekar Nilesh Suryakant

Department of Genetics and Plant Breeding, Lovely Professional University, Punjab, India.

*Author to whom correspondence should be addressed.


A mutation is an abrupt, heritable alteration in a living cell's DNA that is not brought about by genetic recombination or segregation. The deliberate use of mutations in plant breeding is known as "mutation breeding." Mutation breeding provides the advantage of improving a fault in an otherwise excellent cultivar without sacrificing its agronomic and qualitative features, in contrast to hybridization and selection. There is no simpler solution than mutation breeding to enhance seedless crops. These benefits have led to the development of a market for mutation breeding in plant breeding since the initial release of mutant cultivars derived from fundamental mutation research in Europe. Both physical and chemical mutagens have improved methods for inducing mutations in major crops, and strategies for selecting mutant populations have been detailed. A broad range of mutations that have not been previously documented have been detected, and new mutagenic factors like cosmic rays and ion beam radiation are being studied. However, ionising radiation and alkylating chemicals continue to be widely used. The efficiency of mutant breeding has increased as a result of the advent of reliable in vitro methods for numerous crop species. In vitro methods are particularly effective because they can manage sizable mutagenized populations in a small area, have a quicker progeny turnover rate in vegetatively propagated species, and can screen for a variety of biotic and abiotic stress factors in the culture environment. Over the last ten years, there have been significant advancements in mutant screening, with reverse genetic methods now being prioritised. Thus, the combination of molecular methods and mutation techniques is opening up new and intriguing possibilities for contemporary plant breeding.

Keywords: Mutation breeding, agriculture, modern breeding, mutagenesis, crop improvement

How to Cite

Ali, Shahzeb, and Talekar Nilesh Suryakant. 2024. “Mutation Breeding and Its Importance in Modern Plant Breeding: A Review”. Journal of Experimental Agriculture International 46 (7):264-75.


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Iqbal J, Yousaf U. Anther Culturing a Unique Methodology in Achieving Homozygosity. Asian Journal of Advances in Agricultural Research. 2018;8(2):1–9. Available:

Itoh Y. Characterization of Heading Times and Duration of Heading Time of an Individual Using a Wide Range of Variety of Rice (Oryza sativa L.) in One of the Northern-limit Regions of Rice Cultivation, Hokkaido Islands. Journal of Agriculture and Ecology Research International. 2018;8(4):1–9. Available:

Toker C, Yadav SS, Solanki IS. Mutation breeding. Lentil: an ancient crop for modern times. 2007:209-24.

Koornneef M, Meinke D. The development of Arabidopsis as a model plant. The Plant Journal, 2010;61(6):909-921.40.

Olm MR. Strain-resolved metagenomic analysis of the premature infant microbiome and other natural microbial communities. University of California, Berkeley; 2019.

Redei GP. Dedication: Ernest Robert Sears (1910–1931) geneticist par excellence, cytogeneticist extraordinaire, and a good man. Plant Breed. Rev. 1992;10:1–22.

Ashikari M, Sasaki A, Ueguchi-Tanaka M, Itoh H, Nishimura A, Datta S, Ishiyama K, Saito T, Kobayashi M, Khush GS, Kitano H, Matsuoka M. Loss-of-function of a rice gibberellin biosynthetic gene, GA20 oxidase (GA20ox-2), led to the rice “green revolution.” Breed. Sci. 2002;52:143–150.

Jehan T, Lakhanpaul S. Single nucleotide polymorphism (SNP)-methods and applications in plant genetics: a review. Indian J. Biotechnol. 2006;5:435–459.

Lee S, Costanzo S, Jia Y. The structure and regulation of genes and consequences of genetic mutations. In: Q.Y. Shu, B.F. Forster, and H. Nakagawa (eds.), Plant mutation breeding and biotechnology. CABI, FAO, Oxfordshire, UK, Rome. 2012;31–46.

Lisch D. How important are transposons for plant evolution? Nature Rev. Genet. 2013;14:49–61.

Mussgnug JH, Nuclear Transformation and Toolbox Development. In Chlamydomonas: Molecular Genetics and Physiology. Springer, Cham. 2017;27-58.

Prina AR, Pacheco MG, Landau AM. Mutation induction in cytoplasmic genomes. In: Q.Y. Shu, B.F. Forster, and H. Nakagawa (eds.), Plant mutation breeding and biotechnology. CABI, FAO, Oxfordshire, UK. 2012;203–208.

Fluhr R, Aviv D, Galun E, Edelman M. Efficient induction and selection of chloroplast-encoded antibiotic-resistant mutants in Nicotiana. Proc. Natl. Acad. Sci. USA. 1985;82:1485–1489.

Bowman KD, Gmitter FG, Moore GA, Rouseff RL. Citrus-fruit sector chimeras as a genetic resource for cultivar improvement. J. Am. Soc. Hort. Sci. 1991;116:888–893.

Corley RHV, Lee CH, Law IH, Wong CY. Abnormal flower development in oil palm clones. Planter. 1986;62:233–240.

Jaligot E, Adler S, Debladis E, Beule T, Richaud F, Ilbert P, Finnegan EJ, Rival A. Epigenetic imbalance and the floral developmental abnormality of the in vitro regenerated oil palm Elaeis guineensis. Ann. Bot. 2011;108:1453–1462.

Sparrow AH, Singleton WR.The use of radiocobalt as a source of gamma-rays and some effects of chronic irradiation on growing plants. Am. Nat. 1953;87:29–48.

Magori S, Tanaka A, Kawaguchi M. Physically induced mutation: ion beam mutagenesis. In: K. Meksem and G. Kahl (eds.), The handbook of plant mutation screening. Wiley-VCH Verlag GmbH, Weinheim, Germany. 2010;1–16.

Singh H, Khar A, Verma P. Induced mutagenesis for genetic improvement of Allium genetic resources: a comprehensive review. Genetic Resources and Crop Evolution. 2021;1-22.

Stadler LJ. Mutations in barley induced by X-rays and radium. Science. 1928b; 68:186–187.

Esnault MA, Legue F, Chenal C. Ionizing radiation: advances in plant response. Environ. Expt. Bot. 2010;68:231–237.

Lagoda PJL.. Effects of radiation on living cells and plants. In: Q.Y. Shu, B.F. Forster, and H. Nakagawa (eds.), Plant mutation breeding and biotechnology. CABI, FAO, Oxfordshire, UK. 20121;23–134.

Briggs RW, Constantin MJ. Radiation types and radiation sources. In: Manual on mutation breeding, 2nd ed., IAEA, Vienna. Tech. Rep. Ser. 1977;119:7–20.

Donini P, Sonnino A.. Induced mutation in plant breeding: current status and future outlook. In: S.M. Jain, D.S. Brar, and B.S. Ahloowalia (eds.), Soma clonal variation and induced mutations in crop improvement. Kluwer Academic Publ., Dordrecht, UK.1998;255–291.

Heslot H. Review of main mutagenic compounds. In: Manual on mutation breeding, 2nd ed., 2nd edn., pp. 51–59. IAEA, Vienna. Tech. Rep. Ser. 1977; 119.

Minocha JL, Arnason TJ. Mutagenic effectiveness of ethyl methane sulphonate and methyl methane sulphonate in barley. Nature. 1962;196:499.

Leitão JM. Chemical mutagenesis. In: Q.Y. Shu, B.F. Forster, and H. Nakagawa (eds.), Plant mutation breeding and biotechnology. CABI, FAO, Oxfordshire, UK2012;135–158.

Tadele Z, Mba C, Till BJ. TILLING for mutations in model plants and crops. In: S.M. Jain and D. S. Brar (eds.), Molecular techniques in crop improvement (2nd ed). Springer, The Netherlands.2009;307 –332.

Bezie Y, Tilahun T, Atnaf M, Taye M. The potential applications of site-directed mutagenesis for crop improvement: A review. Journal of Crop Science and Biotechnology. 2021;24(3):229-244.

Kleinhofs AC. Sander RA. Nilan, Konzak CF. Azide mutagenicity mechanism and nature of mutants produced. In: Proceedings polyploidy and induced mutations in plant breeding. 1972. IAEA, Vienna. 1974;159–199.

Aastveit K. Effects of combinations of mutagens on mutations frequency in barley.. In: Mutations in plant breeding II. IAEA, Vienna. 1968;5–14

Azpiroz-Leehan R, Feldmann KA. T-DNA insertion mutagenesis in Arabi dopsis: going back and forth. Trends Genet. 1997;13:152–156.

McCarty D, Meeley R.. Transposon resources for forward and reverse genetics in maize. In: J. Bennetzen and S. Hake (eds.), Handbook of maize. Springer, New York. 2009;561–584.

Zong X, Yang T, Liu R, Zhu Z, Zhang H, Li L, Zhang X, He Y, Sun S, Liu Q. Li G,. Genomic designing for climate-smart pea. In Genomic designing of climate-smart pulse crops. Springer, Cham. 2019;265-358.

Anne S. Lim JH. Mutation breeding using gamma irradiation in the development of ornamental plants: A review. Flower Research. 2020;28(3):102-115.

Germana MA. Use of irradiated pollen to induce parthenogenesis and haploid production in fruit crops.. In: Q.Y. Shu, B. P. Forster, and H. Nakagawa (eds.), Plant mutation breeding and biotechnology. CABI, FAO, Oxfordshire, UK. 2012;411–421.

Greene EA, Codomo CA, Taylor NE, Henikoff JG, Till BJ, Reynolds SH, Enns LC, Burtner C, Johnson JE, Odden AR, Comai L, Henikoff S. Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis. Genetics 2003;164:731– 740.

Hirochika H, Guiderdoni E, An G, Hsing Y.i, Eun M, Han Cd, Upadhyaya N, Ramachandran S, Zhang Q, Pereira A, Sundaresan V, Leung H. Rice mutant resources for gene discovery. Plant Mol. Biol. 2004;54:325–334. Available:!Search

Jankowicz-Cieslak J, Kozak-Stankiewicz K, Seballos G, Razafinirina L, Rabefiraisana J, Rakotoarisoa NV, Forster B, Vollmann J. Till BJ. Application of soft X-ray and near-infrared reflectance spectroscopy for rapid phenotyping of mutant rice seed. In: Proceedings transformation technologies (plants and animals), Plant Gen. Breed. Technol. Vienna. 2013;37–40.

Kumar A. The adventures of the Ty1-copia group of retrotransposons in plants. Trends in Genet. 1996;12:41 –43.

Monson-Miller J, Sanchez-Mendez DC Fass J, Henry IM, Tai TH, Comai L. Reference genome-independent assessment of mutation density using restriction enzyme-phased sequencing. BMC Genomics. 2012;13:72–86.

Ossowski S, Schneeberger K, Lucas-Lledo JI, Warthmann N, Clark RM, Shaw RG, Weigel D, Lynch M. The rate and molecular spectrum of spontaneous mutations in Arabidopsis thaliana. Science. 2010;327:92–94.