Enhancing Juvenile Nile Tilapia Growth and Health through Prebiotic and Probiotic Supplementation: A Comprehensive Study
Jaqueline Murbach Braz *
Universidade Federal da Grande Dourados (UFGD), Brazil.
Arypes Scuteri Marcondes
Universidade Federal da Grande Dourados (UFGD), Brazil.
Matheus Antonio do Amaral
Universidade Federal da Grande Dourados (UFGD), Brazil.
Rayane Seibt Moraes
Universidade Federal da Grande Dourados (UFGD), Brazil.
Weliton Vilhalba
Universidade Federal da Grande Dourados (UFGD), Brazil.
Agnês Markiy Odakura
Universidade Federal da Grande Dourados (UFGD), Brazil.
Claucia Aparecida Honorato
Universidade Federal da Grande Dourados (UFGD), Brazil.
Leonardo de Oliveira Seno
Universidade Federal da Grande Dourados (UFGD), Brazil.
Dacley Hertes Neu
Universidade Federal da Grande Dourados (UFGD), Brazil.
*Author to whom correspondence should be addressed.
Abstract
This study investigates the synergistic effects of prebiotics and probiotics on the productivity and physiological parameters of juvenile Nile tilapia (Oreochromis niloticus). Conducted over 60 days at the Federal University of Grande Dourados, the experiment utilized a completely randomized design with 300 fish distributed across five treatments: Control, PROB (4 g.kg-1), PREB MOS (4 g.kg-1), SIMB I (2 g.kg-1 probiotic + 2 g.kg-1 prebiotic), and SIMB II (4 g.kg-1 probiotic + 4 g.kg-1 prebiotic). Key findings include improved feed conversion, protein efficiency rate, and protein retention in treatments with additives, with SIMB II showing the highest intestinal quotient and plasma glucose index. Histological evaluations of the midgut and enzymatic analysis of the liver and intestine showed no significant differences between treatments. Multivariate analysis revealed distinct physiological responses in fish receiving additives compared to the control group. The use of MOS additives and probiotics improved both the development and health status of Nile tilapia juveniles (Oreochromis niloticus) throughout their growth. The use of the SIMB II dose (4 g.kg-1 of probiotic + 4 g.kg-1 MOS) is recommended it should be changed as Throughout their growth, the development and health status of juvenile Oreochromis niloticus tilapia. It is advised to use the SIMB II dosage (4 g.kg-1 of probiotic + 4 g.kg-1 MOS).
Keywords: Biotechnology, non-pathogenic bacteria, additives, gut health