Evaluation of Trichoderma Isolates for Management of Soybean Anthracnose Caused by Colletotrichum truncatum
Akshay Kumar Khare
*
Department of Plant Pathology, JNKVV, College of Agriculture, Jabalpur (M.P.), India.
Ashish Kumar
Department of Plant Pathology, JNKVV, College of Agriculture, Jabalpur (M.P.), India.
Stuti Sharma
Department of Plant Breeding and Genetics, JNKVV, College of Agriculture, Jabalpur (M.P.), India.
Anurag Chouhan
Department of Plant Pathology, JNKVV, College of Agriculture, Jabalpur (M.P.), India.
Abhijeet Kumar Dubey
Department of Agronomy, JNKVV, College of Agriculture, Jabalpur (M.P.), India.
Nishi Mishra
Seed Technology Research Center, JNKVV, Jabalpur (M.P.), India.
Prashant Namdeo
Seed Technology Research Center, JNKVV, Jabalpur (M.P.), India.
Ravi Kumar Dubey
Directorate of Research Services, JNKVV, Jabalpur (M.P.), India.
Sakshi Mishra
Department of Horticulture, JNKVV, College of Agriculture, Jabalpur (M.P.), India.
Pallavi Dhurwey
Department of Plant Pathology, JNKVV, College of Agriculture, Jabalpur (M.P.), India.
Ashutosh Chauhan
Department of Plant Pathology, JNKVV, College of Agriculture, Jabalpur (M.P.), India.
Muskan Deshmukh
Department of Plant Pathology, JNKVV, College of Agriculture, Jabalpur (M.P.), India.
*Author to whom correspondence should be addressed.
Abstract
Soybean anthracnose, caused by Colletotrichum truncatum, is a major constraint to soybean productivity worldwide. The present study evaluated the antagonistic potential of eight Trichoderma isolates against C. truncatum under both In-vitro and in-vivo conditions to identify effective biocontrol agents for sustainable disease management. In-vitro screening, conducted using the dual culture technique on Potato Dextrose Agar (PDA) in a Completely Randomized Design (CRD) with nine treatments (including one untreated control), revealed significant inhibition of pathogen growth by all isolates. Among them, T. reesei-1 exhibited the highest mycelial inhibition (55.56%), followed by T. harzianum-2 (54.70%), T. harzianum-1 (51.71%), and T. reesei-3 (49.57%). In-vivo evaluation under greenhouse conditions showed a marked reduction in anthracnose incidence, with T. reesei-1 recording the lowest disease incidence (6.25%) compared to 76.25% in the pathogen control. The strong antagonistic effect of these isolates may be attributed to the production of antifungal metabolites and efficient root colonization. Overall, the study highlights T. reesei-1 and T. harzianum-1 as promising bioagents for the eco-friendly and sustainable management of soybean anthracnose.
Keywords: Soybean, Colletotrichum truncatum, trichoderma, anthracnose, biocontrol