Plant Regeneration from Shoot Tips-derived Callus of Ginger (Zingiber officinale Rosc.)
Diaa A. Ibrahim *
Department of Scientific Research Center, Faculty of Science, University of Duhok, Iraq
Gharbia H. H. Danial
Department of Scientific Research Center, Faculty of Science, University of Duhok, Iraq.
Vian M. Mosa
Department of Scientific Research Center, Faculty of Science, University of Duhok, Iraq
Belan M. Khalil
Department of Scientific Research Center, Faculty of Science, University of Duhok, Iraq.
*Author to whom correspondence should be addressed.
Abstract
A protocol was described to regenerate plants from callus culture of ginger (Zingiber officinale Rosc.). Callus cultures were induced from ginger shoot tips inverted cultured on semi-solid MS medium supplemented with 2, 4-D (0.5-1.0 mg L-1) and BA (0.5- 1.0 mg L-1). Maximum callus induction was obtained on MS medium supplemented with BA (0.5 mg L-1) either alone or with 2,4-D (0.5 – 1.0 mg L-1), while the highest callus weight was on MS medium supplemented with BA (0.5 mg L-1) and 2,4-D (0.5 mg L-1). Induced callus was tested for regeneration on MS medium supplemented with different concentrations of 2,4-D and BA and the best regeneration was observed on a medium containing 2.5 mg L-1 BA. The regenerated plants were rooted and successfully established in the field after few days of acclimatization.
Keywords: Ginger, callus induction, plant regeneration, shoot tips