Polymorphism in Some Egyptian Wheat Varieties Based on SSR-Markers
Nader R. Abdelsalam *
Faculty of Agriculture (Saba Pasha), Agricultural Botany Department, Alexandria University, Alexandria, Egypt. and Kansas State University, Agronomy Department, USDA Genotyping Center, Manhattan, KS, USA. Article Information
*Author to whom correspondence should be addressed.
Abstract
In this study 312 Microsatellite markers were used to analyze DNA polymorphism of three Egyptian wheat aiming to develop specific molecular markers useful in future Egyptian wheat breeding programs. DNA was extracted using the CTAB method and PCR products were separated in an ABI 3730 DNA analyzer. Data were scored using GeneMarker and 2.5% Agarose gel. A Total of 477 fragments were detected and among 312 simple sequences repeat markers 162 were proved to be polymorphic. The percentage of genetic polymorphism ranged from 33% to 100 % and fragment size from 112 to 535 bp. Results of these experiments consider the first step in the effective detection of polymorphism among some Egyptian wheat varieties in order to correct choose for parents in future.
Keywords: Wheat, MAS, SSR, PCR, polymorphism