In-vitro Acaricidal Study of Calotropis Gigantea Methanolic Extracts against (Rhipicephalus (B.) microplus) Ticks in Udaipur, Rajasthan, India
Anuj Dixit
Department of Veterinary Parasitology CVAS, Navania, Udaipur, India.
Hakim Manzer *
Department of Veterinary Parasitology CVAS, Navania, Udaipur, India.
*Author to whom correspondence should be addressed.
Abstract
The present research was conducted to find out acaricidal efficacy of methanolic extracts of Calotropis gigantea against Rhipicephalus (Boophilus) microplus ticks in Udaipur (Rajasthan). Larval packet Test (LPT) and Adult Immersion Test (AIT) were used to evaluate acaricidal efficacy. A total of four concentrations of the Calotropis gigantea extract (12.5%, 25%, 50%, and 100%) were used along with one control group with twice replications for each concentration were used in the bioassay. The Highest efficacy in both in-vitro tests (AIT and LPT) was recorded in 100% methanolic extracts of Calotropis gigantea. The Calotropis gigantea methanolic extracts displayed the highest IO% at 100% concentration, which was determined to be 67.46% in AIT. The methanolic extracts at 100% concentration had a minimum reproductive index of 0.16. During the study, it was clear that AIT had a lower reproductive index and a higher percent oviposition inhibition. Calotropis gigantea methanolic extracts in LPT demonstrated the highest efficacy at 100% concentration, which was of 64%. The Larval mortality increased trend with increasing concentrations of plant extracts.
Keywords: Calotropis gigantean, ticks, Larval Packet Test (LPT), Adult Immersion Test (AIT)