Journal of Experimental Agriculture International

Aims: The study aimed to verify the potential for growth promotion of five bacterial strains to determine the best period to verify the interaction between plant and bacteria.

Study Design: The experimental design was completely randomized and the analyzes were performed at seven and fourteen days after the inoculation.

Place and Duration of Study: The experiment was performed at the biotechnology lab of the Pontifical Catholic University of Paraná, Campus Toledo.

Methodology: The strains Azospirillum brasiliense, Herbaspirillum sropedicae, Pantoea ananatis, Burkholderia ambifaria and Burkholdeira sp., were tested to growing promotion on simple hybrid corn 30F53 YH being the inoculation made in pre-germinated seeds with 10⁶ CFU mL^-1 posteriorly the same seeds were kept on genobiotic conditions with N restriction. Were evaluated the parameters aerial and root fresh weight, aerial length, root length, N percentage on aerial part, root morphology and epiphytic and endophytic population

Results: It was observed that to the seven or even the fourteen days was possible to verify the interaction between plant and bacteria by means of the parameters evaluated in other words in both periods were significant differences between treatments.

Conclusion: Bacteria were not very efficient to promising grow on the hybrid 30F53 YH in vitro on N restriction conditions since no treatment statistically overcame the control to the evaluated parameters.